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TRUE5

Sigma-Aldrich

TrueGel3D Hydrogel Kits

FAST-PVA, PEG non cell-degradable crosslinker

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shipped in

dry ice

storage temp.

−70°C

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General description

The gel is formed by stable thioether bonds between maleimide groups of PVA (FAST-PVA) and thiol groups on PEG non cell-degradable crosslinker. Malemide-functionalized PVA is specifically used to decrease the time for gel formation. The PVA polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.

Application

TRUE5-1KT is preferred over dextran-based gels for long-term culture of cells which producing carbohydrate degrading enzymes. It is used to culture spheroids which proliferate but do not attach to hydrogel. PVA-PEG hydrogel kits are also used to immobilize cells or specimens for imaging.

Features and Benefits

  • Fast gelling (takes few seconds to minutes after addition of crosslinker)
  • Flexibility in modulating gel stiffness
  • Does not allow cell migration
  • Cell inert hydrogel (if not customized with RGD peptide)
  • Cells cannot be recovered

Components

TRUE5-1KT contains FAST-PVA (polymer) in solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol and water.

Caution

Avoid prolong exposure of PEG non cell-degradable crosslinker to air and store Mal-PVA in small portions to avoid frequent freeze-thaw cycles.

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Fibroblasts that are present in the stroma secrete glycoproteins and polysaccharides to form extracellular matrix. They maintain the structural integrity within the connective tissue and play a key role in cancer, chronic obstructive pulmonary diseases, idiopathic pulmonary fibrosis, scleroderma and several other diseases.

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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