WGA4

GenomePlex^® Single Cell Whole Genome Amplification Kit

Amplify genome of a single cell

• Synonym(s): Single cell whole genome amplification, Whole genome amplification
• EC Number: 254-457-8
• UNSPSC Code: 12352200
• NACRES: NA.55

Properties

• Quality Level: 200
• technique(s): whole genome amplification: suitable
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

GenomePlex^® Single Cell Whole Genome Amplification Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. This kit is designed to amplify the genome of a single cell. This rapid and straightforward method provides millionfold amplification yielding microgram quantities of genomic DNA from a single cell. Traditional single-cell whole genome amplification methods yield insufficient quantities with significantly biased representation. The kit includes all the reagents necessary for cell lysis and successive whole genome amplification. Single cells can be isolated by fluorescence-activated cell sorting (FACS), laser capture microdissection (LCM), dilution, or any other applicable method.

Application

GenomePlex^® Single Cell Whole Genome Amplification Kit has been used:

• to amplify the isolated DNA[1]
• in whole genome amplification (WGA)[2]
• to amplify the microdissected DNAs[3]
• for the amplification of circulating tumor cell genomic DNA from metastatic castration-resistant prostate cancer cells[4]
• for the detection of copy number variations (CNV) by single-cell low-coverage whole-genome sequencing (SLWGS) method[5]
• Gel electrophoresis
• qPCR
• comparative genomic hybridization (CGH) microarray
• Short tandem repeats (STR) analysis
• single nucleotide polymorphism (SNP) analysis

Features and Benefits

• Highly yield and accuracy of DNA amplification within four hours
• Amplification of DNA from any source such as cancer cells, epithelial cells, lymphocytes, fibroblast amniotic cells, polycarbonate fixed cells, and plant cells
• A complete representation of the entire genome with minimal allele bias
• WGA DNA polymerase is suitable for use with downstream applications including gel electrophoresis, qPCR, comparative genomic hybridization (CGH) microarray, short term repeat (STR) analysis, and single nucleotide polymorphism (SNP) analysis, TaqMan^® assays, and microsatellite analysis

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

GenomePlex is a registered trademark of Takara Bio USA, Inc.

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Kit Components Also Available Separately

• L1043 10x Single Cell Lysis & Fragmentation Buffer
• P4850 Proteinase K from Tritirachium album, buffered aqueous glycerol solution, Molecular Biology, ≥800 units/mL
• W4502 Water, Nuclease-Free Water, for Molecular Biology

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable