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Y-27632 dihydrochloride

≥98% (HPLC)

(R)-(+)-trans-4-(1-Aminoethyl)-N-(4-Pyridyl)cyclohexanecarboxamide dihydrochloride
Empirical Formula (Hill Notation):
C14H21N3O · 2HCl
CAS Number:
Molecular Weight:
MDL number:
PubChem Substance ID:

biological source

synthetic (organic)

Quality Level


≥98% (HPLC)



optical activity

[α]/D +3.0 to +5.0°, c = 1.0 in methanol


white to beige


H2O: 14 mg/mL

storage temp.


SMILES string




InChI key


Gene Information

General description

Y-27632 dihydrochloride is an effective bronchodilator and improves lung resistance (RL) induced by antigen and acetylcholine. Y-27632 controls neutrophil migration and lung edema and may be a potential drug for treating acute lung injury.


Y-27632 dihydrochloride has been used:
  • as a medium supplement in pancreatic ductal adenocarcinoma organoid culture
  • in the inhibition of Ras homolog gene family (Rho) kinase in mouse embryonic stem cells
  • in the inhibition of Rho-associated protein kinase (ROCK) in human embryonic stem cells and human induced pluripotent stem cells (iPSCs)


1, 5 mg in glass bottle

Biochem/physiol Actions

Y-27632 is highly potent, cell-permeable, selective ROCK (Rho-associated coiled coil forming protein serine/threonine kinase) inhibitor. Ki = 140 nM for p160ROCK. Y-27632 also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP.

Features and Benefits

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Legal Information

Sold under license from Mitsubishi Pharma Corporation

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Attenuation of endotoxin-induced acute lung injury by the Rho-associated kinase inhibitor, Y-27632
Tasaka S, et al.
American Journal of Respiratory Cell and Molecular Biology, 32(6), 504-510 (2005)
Human cardiomyocytes undergo enhanced maturation in embryonic stem cell-derived organoid transplants
Varzideh F, et al.
Biomaterials, 192, 537-550 (2018)
Basement membrane destruction by pancreatic stellate cells leads to local invasion in pancreatic ductal adenocarcinoma
Koikawa K, et al.
Cancer Letters, 425, 65-77 (2018)
Evaluation of Y-27632, a rho-kinase inhibitor, as a bronchodilator in guinea pigs
Iizuka K, et al.
European Journal of Pharmacology, 406(2), 273-279 (2000)
Naoki Okumura et al.
Investigative ophthalmology & visual science, 55(1), 318-329 (2013-10-10)
To investigate the molecular mechanism of Rho-associated kinase (ROCK) inhibitors Y-27632 and Y-39983 on corneal endothelial cell (CEC) proliferation and their wound-healing effect. The expression of G1 proteins of the cell cycle and expression of phosphorylated Akt in monkey CECs


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