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59356-U

Discovery® Cyano (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Synonym(s):

Cyano Bonded Phase Column

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Size/SKUAvailabilityPrice
1 ea

Available to ship TODAYfromToluca

MXP 28,969.00

About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
15 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
CN, cyano phase
Pore size:
180 Å
Matrix:
silica gel, high purity, spherical base material, fully porous particle

MXP 28,969.00

Available to ship TODAYDetails

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Technical Service
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Product Name

Discovery® Cyano HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Quality Segment

agency

suitable for USP L10

product line

Discovery®

feature

endcapped

analyte functional class(es)

explosives, oils

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

4.5% Carbon loading

parameter

≤70 °C temp. range, 400 bar pressure (5801 psi)

technique(s)

HPLC: suitable, LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

surface area

200 m2/g

surface coverage

3.5 μmol/m2

impurities

<10 ppm metals

matrix

silica gel, high purity, spherical base material, fully porous particle

matrix active group

CN, cyano phase

particle size

5 μm

pore size

180 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

General description

Discovery® Cyano HPLC column is used for the retention and separation of strongly basic analytes including quaternary ammonium salts, with excellent peak shapes.

Application

Discovery Cyano HPLC columns provide a lower hydrophobicity than C18 or C8 and different selectivity. Typical applications are the separation of Flavonoids, Phenols, Phthalates, Steroids, Sulfonamides, Oils and Alcohols.

Features and Benefits

  • Low hydrophobicity for rapid elution of hydrophobic analytes
  • Excellent peak shape and retention of strongly basic analytes
  • Retention of polar analytes
  • Unique selectivity
  • Significantly less retention than C18 (typically requires lower % organic mobile phase)
  • Stable, low-bleed LC-MS separations
  • Compatible with highly aqueous organic phases

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

13 - Non Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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Certificates of Analysis (COA)

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Questions

1–6 of 6 Questions  

  1. What is the shipping solvent for column Discovery® Cyano HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

    1 answer

    1. The shipping solvent for this product and all Discovery® Cyano HPLC Columns is acetonitrile.

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  3. Pore volume of stationary phase of Discovery® Cyano HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm (Part No: 59356-U)

    1 answer

    1. For this product, the pore diameter is 180 Å, the particle size is 5 μm, and the surface area is 200 m²/g. However, the pore volume is not a tested parameter.

      Helpful?

  4. ¿Me podrías confirmar que la composición de esta columna es end-capped cyanosilyl silica gel for chromatography R? Gracias!

    1 answer

    1. These columns are end-capped, but the phase is cyanopropyl.

      Helpful?

  5. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  6. Please provide the below details for the column Carbon loading%- Pore size- Surface area- pH stability- End capping-

    1 answer

    1. TS Answer:
      - 4.5% Carbon loading
      - Pore size: 180 Å
      - Surface area: 200 m2/g
      - Operating pH range: 2-8
      - Endcapped
      - Particle Size: 5 μm
      - Temperature Range: ≤70 °C
      - Maximum Pressure: 400 bar pressure (5801 psi)
      - Suitable for USP L10

      Helpful?

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Reviews

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  1. India
    • Review 1
    • Votes 0
    2 out of 5 stars.

    Column to column variation

    Variation in Retention time from column to column observed

    Helpful?

    1. Response from Merck KGaA:

      Thank you for your review. While minor variability between batches may occur, the degree of difference in retention times would be impacted by run times. Assuming all other conditions are identical, an overall +/- 5% may be possible. As there are a number of variables to consider, we encourage customers who experience problems with our products to call or email us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.