Solid phase microextraction (SPME) is a versatile and solvent-free sample preparation technique. It works on the principle of adsorption/absorption and desorption and uses a fiber coated with an extractive phase to concentrate analytes in a sample. It successfully combines the sampling, isolation, and enrichment of an analyte to one step. SPME is used widely for the sampling of environmental volatiles, food & beverages, flavors & fragrances, forensics & toxicology, and pharmaceutical samples.
Selection Guide for Supelco SPME Fibers
The use of SPME-GC/MS to determine the best packaging material for preventing light-induced off-flavors in milk
Rugged and Reproducible Solid Phase Microextraction (SPME) Sampling with Nitinol-Core Fibers
Brochure: SPME for GC Analysis
Brochure: Supel™ BioSPME 96-Pin Devices
Application Note: Sample Preparation of Serum Prior to Determination of Free Testosterone
The smart SPME fibers are an extension of our expertise and innovation from traditional SPME coatings to smart SPME format for accurate, reliable, and consistent results. Our smart fibers including Carboxen® fibers, dual coated fibers, or overcoated fibers are fitted with a unique chip to offer seamless sample preparation and other including benefits:
See for more information on Smart SPME Fibers for Automated Sampling.
The nitinol-core of SPME fibers enhances the fiber performance and the precision of chromatographic results. Fibers with either Carboxen®/Polydimethylsiloxane (CAR/PDMS) or Polydimethylsiloxane/ Divinylbenzene (PDMS/DVB) coatings are available with a nitinol-core. Other benefits of nitinol fibers include:
See for more information on Nitinol Core SPME Fibers.
The over coating of fibers is a new technology in fiber coating to protect the SPME fiber from matrix components. This allows for immersion of the fiber directly into complex samples ensuring a more efficient extraction (e.g. of semivolatile compounds). Polydimethylsiloxane (PDMS) is used to overcoat a PDMS/divinylbenzene (DVB) fiber. The resulting fiber is more physically robust and resistant to chemical fouling and allows for an efficient wash step to also remove adhering matrix.
BioSPME technology greatly improves upon the speed and simplicity compared with current bioanalytical sample preparation techniques employed for plasma protein binding studies and other free analyte analyses. The primary benefits of Supel™ BioSPME devices include:
See for more information on Supel™ BioSPME 96-Pin Devices.