Cultured cells require a supply of nutrients for growth and differentiation. Mammalian cell culture media contains a balance of pH, salts, carbohydrates, amino acids, vitamins, fatty acids, lipids, growth factors, hormones, trace elements, and serum. Hormones promote cell growth and can act in a cell-type specific manner. Some cells require hormone supplements to grow under in vitro culture.
Cell Culture Grade Hormone Supplements
- The hormone dexamethasone is used in cell culture to study apoptosis, cell signaling pathways, cell differentiation, and gene expression.
- Erythropoietin promotes the proliferation, differentiation, and survival of erythroid progenitors.
- β-Estradiol hormone is used to study cell differentiation and transformation. β-Estradiol has been used for culturing mammary tumor cells and oocytes.
- Glucagon is a peptide hormone that is used as a supplement in some culture and animal model applications, such as measuring glucagon response in mice.
- Hydrocortisone can be used in epithelial and endothelial adherent cell culture applications.
- Insulin is a peptide hormone that is used as a growth factor in many mammalian cell culture systems.
- Progesterone has been used as a growth-promoting hormone in epithelial cell culture.
- Prostaglandin E1 and prostaglandin E2 are lipid hormones that are used in some mammalian cell culture applications.
- 3,3′,5′-Triiodo-L-thyronine (T3) and its precursor thyroxine (T4) hormones are produced by the thyroid gland and are used as supplements in some cell culture applications.