Transfection Reagents
What are the reagents used in transfection? Transfection is the process of introducing genetic material and molecules, including DNA, RNA, and proteins into eukaryotic cells for gene expression, gene knockdown, and many other applications. Transfection uses various physical, chemical, or biological methods and reagents to facilitate the transfer of the foreign material. Comparatively, bacterial transformation and viral transfection (viral transduction) are similar techniques that are suitable for transferring materials into different cell types for diverse downstream applications. For incremental innovations, day after day, choose transfection reagent essentials that are the foundation of great work, not a variable that undermines it.
With our acquisition of Mirus Bio, we are proud to offer a significant expansion of our broad-range transfection solutions, viral vector production enhancers, and numerous molecular reagents that reliably take your workflow from the bench to the bioreactor. Through this partnership, we reaffirm our commitment to supporting our customers in advancing cell and gene therapies. With trusted products that support the delivery of nucleic acids into various cell types, including TransIT® transfection reagents and kits, we now offer Mirus Bio’s world-class product offerings and scientific experience. Mirus Bio products will be added to our product selection offerings soon. However, you can easily discover the best Mirus Bio Reagents for your ground-breaking discoveries and step toward advancing your next-generation therapy on the Mirus Bio platform now.
Products
Dimethyl sulfoxide
Molecular Biology
DAPI
for nucleic acid staining
X-tremeGENE™ HP DNA Transfection Reagent
High-performance polymer reagent for transfecting many cell lines
X-tremeGENE™ 9 DNA Transfection Reagent
Polymer reagent for transfecting common cell lines
G-418 Solution
solution, =98% (TLC), suitable for transfection
Hygromycin B
from Streptomyces hygroscopicus
X-tremeGENE™ 360 Transfection Reagent
Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines
DAPI ready made solution
For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL
DOTAP Liposomal Transfection Reagent
>99% (TLC), liquid, suitable for transfection
X-tremeGENE™ siRNA Transfection Reagent
Polymer reagent for delivering siRNA to common cell lines
DAPI ready made solution with Antifade
For Nuclear counterstain in Fluorescence Microscopy
Escort™ IV Transfection Reagent
Lipid reagent for transient and stable transfection of mammalian and insect cells.
Calcium Phosphate Transfection Kit
Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells
Escort™ III Transfection Reagent
Lipid reagent for transfecting sensitive and primary cells
BioPORTER® QuikEase™ Protein Delivery Kit
for intracellular delivery
NeuroPorter™ Transfection Kit
Lipid formulation for nucleic acid transfections in neuronal and glial cells
α-Tri-Calcium phosphate
Reagent for transient & stable DNA transfections
TransIT®-2020
A high performance, animal-free, broad spectrum DNA transfection reagent
TransIT®-X2
A novel, polymeric system for delivery of multiple nucleic acids to mammalian cells
TransIT®-293
A high efficiency, low toxicity, DNA transfection reagent optimized for 293 cells
Calcium Phosphate, Lipid and Polymer-based Transfection Reagents for Delivering DNA, siRNA, miRNA, and CRISPR/Cas Components
The introduction of plasmid DNA or RNA into mammalian and insect cells has become routine in research labs. High-efficiency transfection in nearly any cell type can be achieved thanks to the wide variety of reagents that are currently available. We cover a variety of popular transfection reagents, including our Escort™ lipid reagents, NeuroPorter™ neuronal-specific reagent, X-tremeGENE™, and Genejuice transfection reagents. For help selecting the right reagent for your cells, explore our wide selection of transfection reagents within the product selection table or our Molecular Cloning and Protein Expression resource page for related products.
X-tremeGENE™ Roche Transfection Reagents
With a joint commitment to enable your next discovery, we exclusively offer the complete portfolio of Roche X-tremeGENE™ transfection reagents. Roche, X-tremeGENE™, transfection reagents provide efficient delivery of DNA, siRNA, miRNA, and CRISPR/Cas9 components for common and difficult-to-transfect cell lines. The X-tremeGENE™ products are easy to optimize for your application needs and produce minimal toxicity.
Related Resources
- Brochure: Molecular Essentials Guide
Learn how our comprehensive molecular tools and reagents provide precise, ready-to-use solutions for your workflows.
- Flyer: X-tremeGENE™ 360 Transfection Reagent
Universal polymer transfection reagent for a broad range of applications and cell lines.
- Article: X-tremeGENE™ Transfection Reagents Comparison Guide
Explore our X-tremeGENE™ transfection reagent recommendations guide for the cell type and application best suited for your specific needs.
- Article: Introduction to Transfection vs Transduction and Protocols
Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.
- Article: Blue-White Screening & Protocols for Colony Selection
Learn about and find protocols for the blue white screen technique used in molecular biology research to identify recombinant bacterial clones for further analysis.
- Article: Standard PCR Protocol
Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.
- Article: Western Blotting Protocol
Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.
- Article: Ribonucleoprotein (RNP) Protocols Using Synthetic sgRNAs and Cas9 proteins
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
- Article: Genome Editing in Plants with CRISPR/Cas9
ZFNs and CRISPR/Cas9 advance targeted genome editing, opening new research avenues.
- Gene Editing 101 eBook
Learn how to understand, design and perform CRISPR experiments, as well as design gRNA, choose a Cas9 format, screen with CRISPR, use advanced CRISPR formats, and much more…
- Article: Successful Transduction Using Lentivirus
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
Web Tools
- PCR Master Mix Calculator
Perform calculations for large scale PCR reactions and ensure success of scaled up reactions with this online PCR Master Mix Calculator.
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