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Advanced gene editing

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Lentivirus Cell Line
Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.
CRISPR Essentials: MISSION® CRISPR gRNAs, Cas9 and Related Products
Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments.
Water for Nuclease-sensitive Molecular Biology Studies
Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. Ultrafiltration provides a quicker, safer, and more sustainable alternative to DEPC treatment when preparing RNase-free and DNase-free water.
Methods for Enhancing Lentiviral Transduction Efficiency
Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.
Safe Lentivirus Handling
Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.
Water for pH Measurement and Buffer Preparation
Learn how water impurities can affect pH. Water quality defined as pure water or Type 2 water is recommended to prepare buffer solutions or measure pH.
shRNA Gene Families Sets
MISSION® shRNA Library gene sets mapped by Bioinformatics team for specific cellular pathways.
TRC Design and pLKO.1 Vector FAQs
MISSION® shRNA clones in pLKO.1-Puro vector allow bacterial culture propagation and mammalian cell selection for gene silencing studies.
Cas9-Mediated Recombineering for Kdo2-Lipid A
E. coli CRISPR/Cas-mediated Lambda-Red HR vector system enables gene editing via homology-directed repair.
MISSION® Lentiviral Controls
When conducting shRNA experiments, proper controls are a key element of experimental design. Proper use of controls permits accurate interpretation of knockdown results and provides assurance of the specificity of the observed phenotype. We offer a variety of positive, negative
Long Oligos
Our experience with gene construction and microarray development provides us with insight into the potential difficulties of long oligo synthesis. We have developed techniques to purify long oligos, which are unmatched by other suppliers.
CRISPR-based Gene Activation
Sigma has developed a gene activation system based on a fusion of dCas9 to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300.
Successful Transduction Using Lentivirus
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
Validating CRISPR/Cas9-mediated Gene Editing
After you have performed a CRISPR experiment it is important to determine which gRNAs performed successfully editing. There are many ways to validate CRISPR gene editing experiments. A quick and easy way to check for cutting is by using the
Reverse Transfection of Plasmid DNA
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
MISSION shRNA Lentiviral Transduction
MISSION® shRNA Lentiviral Transduction application data
Nuclease-Free Water at Your Fingertips
An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.
Small Molecule CRISPR Enhancers
Modulation of homology-directed repair (HDR) within the context of CRISPR-genome editing has led to the identification of small molecules that enhance CRISPR-mediated HDR efficiency in various cell types.
Validating CRISPR/Cas9-mediated Gene Editing
Validate CRISPR gene editing experiments easily with Sigma-Aldrich® T7E1 mismatch detection kit, ensuring successful editing.
Water for PCR Techniques
Learn how water impurities can affect PCR and related methodologies. High purity water purified using ultrafiltration is suitable for PCR and related techniques.
Small RNA and miRNA (microRNA) Sequencing
A review on reducing miRNA and other small RNAs sequencing bias with RealSeq®-AC RNA library preparation on the Illumina® RNA sequencing platform.