Merck
Search Within
Applications
Content Type

Calibration, qualification, and validation

Applied Filters:
facet applications:Calibration, qualification, and validation
facet content type:Technical Article
Improving the Chromatographic Separation of DMB-Labeled Sialic Acids for the Comparison of Biosimilars to Reference Materials
A significantly improved HPLC-fluorescence method for DMB-NANA and -NGNA, and application of this method to compare 2 candidate biosimilar therapeutic proteins to their respective RMs.
LC/MS/MS Method for Determination of Glyphosate, AMPA, and Glufosinate in Cereals
Glyphosate and related compounds are measured in oatmeal and infant cereal using ion-exchange polymer-based particles for HPLC and SPE. Low level detection was obtained.
Quantification of Infliximab in Human Serum by LC-MS/MS Using A Full-Length Stable Isotope Labeled Internal Standard
Infliximab, a chimeric monoclonal antibody, is used to treat rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and other autoimmune diseases.
Multiplex Quantification of Infliximab and Adalimumab in Human Serum by LC-MS/MS Using Full-Length Stable Isotope Labeled Internal Standards
Infliximab and Adalimumab monoclonal antibodies (mAbs) are used widely to treat rheumatoid arthritis, psoriatic arthritis, and many autoimmune diseases by binding to tumor necrosis factor-alpha (TNFα) to reduce the inflammatory response.
Columns and Analytical Standards for Protein SEC
Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.
An LC/MS Peptide Standard for Rapid System Suitability Assessment and Retention Time Translation Across LC/MS Platforms
Standards are critical in liquid chromatography/mass spectrometry (LC/MS) based proteomics to ensure optimal and consistent system performance before, during, and after sample analysis.
A Comparison of Solid Phase Extraction (SPE) Products Used to Purify Peanut Paste Extract for Aflatoxin Analysis
Supel Tox AflaZea SPE produced exceptional analyte recoveries with good reproducibility for all aflatoxins while requiring less than half the sample volume required by competing products.HPLC analysis of all spiked samples after SPE cleanup showed that all compared SPE cartridges
HPLC Separation of Ginsenosides from American Ginseng
The HPLC method for ginsenoside separation was optimized using a ginsenoside standard mixture and then applied to a sample of American Ginseng root. The experimental conditions and chromatograms are shown.
High-Performance Thin-Layer Chromatography: A Fast and Efficient Fingerprint Analysis Method for Medicinal Plants
In this article we present several HPTLC applications and analytical standards for ginsenosides.
Hopane Reference Standards for use as Petrochemical Biomarkers in Oil Field Remediation and Spill Analysis
We provide high-quality, relevant hopane reference standards to be used as biomarkers for petrochemical oil field remediation and spill analysis.
New Certified Reference Materials for α‑Lactalbumin, β‑Lactoglobulin A & B, and Lactoferrin
We have developed Certified Reference Materials (CRMs) of fully intact milk proteins that allow accurate testing of the most critical proteins in infant formula; help labs to develop and test infant formula products with high accuracy, while ensuring legal and
Traceable Organic Certified Reference Materials for P-31 qNMR
The use of CRMs for the NMR quantification of phosphorylated organic compounds and metabolites offers several advantages as it is based on a signal comparison of the analyte with an internal or external reference standard.
Improving the Current USP Method for the Analysis of Lansoprazole Drug Substance Using HPLC Columns Based on Fused-Core® Particle Technology
Compendial methods from the USP (United States Pharmacopeia) are widely used in pharmaceutical drug product and raw materials testing. However, not all methods in the USP use modern technologies. In chromatographic methods, it is not uncommon that older brands of
GC Analysis of a 37-Component FAME Mix on Omegawax®
This technical article will discuss GC Analysis of a 37-Component FAME Mix on Omegawax.
Cannabinoid Analysis of Hemp: Developing an Efficient HPLC Method Workflow
Several different HPLC method options are explored for the efficient and time effective analysis of cannabinoids in a sample of hemp flower. The differences between gradient and isocratic analyses are explored, and the two methods are compared in determining the
Melamine Analysis via GC-MS
Sigma-Aldrich presents an article about the analysis of melamine and related compounds in dog food with the use of more economical gas chromatography-mass spectrometry (GC-MS) instrumentation.
Certified Reference Materials for ¹⁹F Quantitative NMR Ensuring Traceability to “The International System of Units” (SI)
In certain cases, 1H qNMR reaches its limits, especially regarding the certification of complex and larger molecules. However, new fields of application often also bring along the presence of heteroatoms, namely 31P and 19F. We therefore introduced 3 CRMs for
Titripac® Sustainable Packaging for Ready-to-Use Certipur® pH Buffers
Learn about our ready-to-Use Certipur® pH Buffer solutions (4 L & 10 L), available in an innovative Titripac® sustainable packaging for a wide pH range of 1 to 12.
Solution with 17 Amino Acids as TraceCERT® Certified Reference Material
Technical article on "Solution with 17 Amino Acids as TraceCERT® Certified Reference Material"
P-I-A-N-O Standards for Detailed Hydrocarbon Analyses
This is an article concerning P-I-A-N-O standards for detailed hydrocarbon analyses.
Quantification of Bitter Acids in Hops by Thin Layer Chromatography
HPTLC separation followed by UV detection of the bitter acid content in various hop samples.
Comparison of QuEChERS Products for the Extraction of Planar Pesticides from Spinach
Supel™ QuE Verde combines a novel carbon with zirconia coated silica (Z-Sep+) to provide an optimum balance between planar pesticide recovery and color removal.
Vitamin D epi-Metabolites Separated with HybridSPE-Phospholipid
This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.
Drinking Water Testing by Ion Chromatography using Ultrapure Water
This study reviews the suitability of ultrapure water for the IC analysis of drinking water. Inorganic ions and disinfectant by-products (DBPs) were assessed.
Calculating Your Working Tolerance: A Guideline
Guideline to calculate your working tolerance - Certified Standard Solutions (CRM)
Elemental Impurities - Certified Reference Materials for ICH Q3D, USP <232> and Ph.Eur. 5.20
Elemental impurities in drug products pose a risk to patient health due to toxicological effects, and their levels should be controlled within acceptable limits. Find analytical methods and materials for impurity assessment.
Cannabis Potency Testing: Method Development and Cost Considerations
An overview of gradient HPLC-based analysis of cannabinoids, optimized for key method parameters such as column temperature, mobile phase pH, and methanol as low-cost organic modifier. Learn more here.
How to Choose the Correct Reference Material Quality Grade
Reference materials, standards and certified reference materials are critical for analytical testing. We can help you to choose the ideal reference material for your application, whether you are using reference materials for calibration or quantitative analysis.
Enzymatic Method for Determining Branched Chain Amino Acids (BCAA Assay)
Assay protocol for the colorimetric detection of Branched Chain Amino Acids (BCAA) in biological samples using the alanine assay kit.
Page 1 of 2