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Protocol Guide: WST-1 Assay for Cell Proliferation and Viability
WST-1 assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for WST-1 reagent preparation and examples of applications. Frequently asked questions and troubleshooting guide for WST-1 assay.
Substrates Procedures
Our General Protease Assay Procedures and Substrates overview.
Enzymatic Assay of Mutanolysin
This procedure may be used for Mutanolysin products.
Enzymatic Assay of Invertase
Enzymatic Assay of Invertase
Enzymatic Assay of Pepsin (3.4.23.1)
This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.
Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)
To standardize a procedure for the enzymatic assay of Diamine Oxidase.
Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver
Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver. This procedure applies to all b-Glucuronidase products that are derived from Helix Pomatia and Bovine Liver. It is the responsibility of trained Analytical Services laboratory personnel to follow
Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).
Enzymatic Assay of Cellulase
To standardize an enzymatic assay procedure of cellulase.
Lentiviral Titer via p24 (viral coat protein) ELISA Assay
Calculate your lentiviral titer with the p24 ELISA assay. Detailed protcol below.
Enzymatic Assay of Neuraminidase
Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.
Assay Procedure for Glycerol Dehydrogenase
Assay Procedure for Glycerol Dehydrogenase
Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
Enzymatic Method for Determining Phenylalanine (Phenylalanine Assay)
Assay protocol for the fluorometric detection of Phenylalanine in biological samples using the phenylalanine assay kit.
IFN-γ ELISpot Assays on MultiScreen® IP
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
Assay Procedure for Lipase
Assay Procedure for Lipase
Enzymatic Assay of Aprotinin
Objective: To standardize a procedure for the enzymatic assay of Aprotinin.
Enzymatic Assay of Laccase (EC 1.10.3.2)
Laccase (EC 1.10.3.2) is a cuproenzyme that oxidizes various types of phenols and similar aromatic compounds aromatic amines with the reduction of molecular oxygen to water, therefore, is used as a biocatalyst.
Enzymatic Assay of Trypsin Inhibitor
This technical article described the Enzymatic Assay of Trypsin Inhibitor.
GST Detection Module with CDNB Enzymatic Assay
This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.
Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride
Protocol for Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride
Recycling Micro-Assay of β-NAD and β-NADH
Objective: To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH
Assay Procedure for Invertase
The appearance of reducing sugars is measured by the modified Fehling-Lehmann-Schoorl method.
Enzymatic Method for Determining Alanine (Alanine Assay)
Assay protocol for the colorimetric/fluorometric detection of Alanine in biological samples using the alanine assay kit.
Enzymatic Assay of Hyaluronidase (3.2.1.35)
This procedure may be used for Hyaluronidase products.
Enzymatic Assay of Cholesterol Oxidase
This procedure applies to products that have a specification for the enzymatic activity of cholesterol oxidase. This assay is NOT to be used to assay Cholesterol Oxidase from Schizophyllum commune (Discontinued Product Number C7274) and from Brevibacterium sp. (Discontinued Product
MTT Assay Protocol for Cell Viability and Proliferation
MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.
Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]
To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.
Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)
This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.
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