Gene expression and silencing

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facet applications:Gene expression and silencing

facet applications:Transfection and gene editing

facet content type:Protocol

Showing 1-30 of 31 results

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

Preparation of Cationic Liposomes & Transfection of Cells - Avanti® Polar Lipids

This procedure describes the Preparation of Cationic Liposomes & Transfection of Cells

Yeast Transformation Kit

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

Procedure for Preparation of Cationic Lipid/Oligomer Complex - Avanti® Polar Lipids

This procedure describes the preparation of Cationic Lipid/Oligomer Complex.

Preparation of the Lentiviral Transduction Particles Using Packaging Plasmid Mix

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

Antibiotic Optimization for Cell Transduction

Determine optimal antibiotic concentration for stable cell lines, ensuring efficient selection in transduced cells.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

Universal Transfection Reagent Protocol

Universal Transfection Reagent enables efficient nucleic acid delivery into various cells, compatible with different cell culture conditions.

Liposome Preparation - Avanti® Polar Lipids

Lipid vesicle preparation involves hydration and sizing steps, applicable regardless of lipid composition.

Simplicon™ RNA Transfection and Electroporation Protocols

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Preparation of Competent Cells and Transformation with pGEX DNA

This page describes preparation of competent cells and transformation with pGEX DNA.

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

siRNA Protocols

MISSION® siRNA Protocols

hESC Transduction Protocol

A detailed protocol for the transduction of human embryonic stem cells using Lentiviral Vectors. Includes product list and references.

MystiCq® MicroRNA® Quantitation System

Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents

Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents

X-tremeGENE™ HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

Biodegradable Nanoparticles

Preparation for biodegradable nanoparticles and their use in transfection protocols .

Lentiviral Production Using X-tremeGENE HP Transfection Reagent

Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.

MISSION® shRNA Guarantee

Guarantee efficient gene knockdown with our shRNA clones, ensuring reliable results for targeted transcript suppression.

Genome Editing in Plants with CRISPR/Cas9

ZFNs and CRISPR/Cas9 advance targeted genome editing, opening new research avenues.

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids for cellular studies in protein interaction, transcription factor, and gene knockdown analyses.

Spinoculation Protocol

Protocol for lentiviral transduction of suspension cells using MISSION TRC shRNA Lentiviral Particles for long-term silencing.

Preparation of Cationic Liposomes & Transfection of Cells - Avanti^® Polar Lipids

This procedure describes the preparation of cationic liposomes and transfection of cells.

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