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Gene expression and silencing

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Safe Lentivirus Handling
Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.
Lentivirus Cell Line
Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.
General Adherent Cells
Protocol for creating create stable cell lines from both dividing and non-dividing cells for use with a lentiviral delivery system.
Ago RIP to Isolate microRNA and their Targets Using Imprint® RNA Immunoprecipitation Kit
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Using Probe-Based Quantitative PCR (Qpcr) to Measure Gene-Level Expression
Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer
Oligonucleotide Quality Control by Mass Spectrometry
Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.
pLKO-1 Vector and shRNA Design Frequently Asked Questions
Frequently asked questions about pLKO-1 vector & shRNA design
T7 RNA Polymerase - recombinant, expressed in E. coli
T7 RNA Polymerase is a DNA-dependant RNA Polymerase that exhibits a very high specificity for the T7 promoter sequence. The polymerase is useful for synthesizing large amounts of RNA suitable for in vitro translation and anti-sense RNA research.
MISSION™ shRNA Library: Next Generation RNA Interference
Work and refinement of the RNAi technology has exploded in recent years. We now know the basic mechanism of the endogenous RNAi pathway, that the pathway is present in most eukaryotes,3 and how cellular machinery can be harnessed to silence
TRC Library Citations
TRC 1.5 human and mouse shRNA libraries
Dual-Labeled Probes
Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.
Glycosaminoglycan Sulfation and Signaling
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
siRNA Delivery in Mammalian Cell Lines
siRNAs are effectively delivered into mammalian cell lines that have been difficult or even impossible to transfect with siRNA when using other commercially available lipofection reagents.
Experimental Design and Analysis — Experimental Design FAQs
We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.
TRC Design and pLKO.1 Vector FAQs
The MISSION® shRNA clones, designed by the TRC, are pre-cloned into the pLKO.1-Puro vector. This lentiviral vector allows for propagation in bacterial culture and selection of inserts in mammalian cells.
esiRNA FAQs (Frequently Asked Questions)
esiRNA are endoribonuclease prepared siRNAs that target the same mRNA sequence for gene silencing. Here are some of the most asked questions regarding esiRNA uses and availability.
General shRNA FAQs
We answers your frequently asked questions about shRNA, the TRC. MISSION and more.
esiRNA Knockdown Efficiency Tested by Western Blotting
Quantitative and qualitative western blotting to validate knockdown by esiRNA.
Molecular Beacons
Discover the power of molecular beacons in genetic research and diagnostics. Learn how these probes are used for real-time detection and monitoring of specific nucleic acid sequences in qPCR, including for in vitro gene expression analysis, genotyping, and more.
Efficient Gene Silencing with MISSION® TRC shRNA
RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.
miRNA (microRNA) Introduction
Mature microRNAs regulate gene expression through various mechanisms; partially complementary to mRNA molecules, downregulating gene expression.
Predesigned siRNA
Rosetta siRNA Design Algorithm optimizes siRNA sequences for reduced off-target effects and increased RNAi performance.
Introduction to Cell Transfection
Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.
Reverse Transcription
One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and
Development of a New Advanced KSOM Mouse Embryo Media that Enables Dual-Use Embryo Culture and Cell Handling Applications
Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.
CRISPR-based Gene Activation
Sigma has developed a gene activation system based on a fusion of dCas9 to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300.
Delivery of Nucleic Acids Using Polymers
Delivery of Nucleic Acids Using Polymers
Successful Transduction Using Lentivirus
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
Analysis of Fluorescent Dyes Used for Labeling DNA in Microarray Experiments
An article concerning Analyzing Properties of Fluorescent Dyes for Labeling DNA in Microarray Experiments presented by Sigma-Aldrich.com
Reverse Transfection of Plasmid DNA
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
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