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Applications:Imaging analysis and live cell imaging
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Microscopic Examination of Samples
This protocol covers 3 modes for the microscopic examination of cell samples.
Immunofluorescence Labeling of Cells
Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.
Protocol Guide: Exosome Labeling Using PKH Lipophilic Membrane Dyes
PKH dyes have been successfully used to label exosomes and extracellular vesicles for in vitro and in vivo tracking experiments. The exosome labeling protocol below details steps for reliable fluorescent labeling of exosomes using PKH dyes for microvesicle tracking experiments.
Washing Microparticles
Microparticles protocol for washing particles may be done via centrifugation. This procedure must be performed carefully.
Cell Capture Imaging Reagent
Immunocytochemistry (ICC) Cell Capture Imaging Reagent and protocol for eliminating wash steps, and is highly effective in applications involving rare cells, low cell count samples, and limited access samples.
Toluidine Blue Staining Protocol for Millicell® Culture Inserts
This is a Toluidine Blue Staining protocol listing materials and methods.