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Bind/Elute vs Flowthrough Mode in Multimodal Chromatography
This page describes the difference between bind/elute and flowthrough modes in multimodal chromatography using Cytiva products.
Affinity Chromatography Troubleshooting
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Capto MMC
Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.
Troubleshooting Guide for Affinity Chromatography of Tagged Proteins
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
Determination of Chlorogenic acid and Baicalin in Compounded Lonucerae Japonicae Flos using a Discovery® HS C18 Column
Extraction and analyses of chlorogenic acid and baicalin active ingredients in traditional Chinese medicine (TCM) according to Chinese pharmacopeia monograph by HPLC with Discovery® C18 column to check: chromatographic data, linearity, specificity and repeatability, LOQ and LOD
HPLC analysis of Letrozole and its impurities by following the USP Guidelines Using a Purospher® STAR RP18e Column and UV Detection
An application note concerning the HPLC analysis of letrozole and its impurities by following the USP43-NF38 Guidelines using a Purospher® STAR RP18e Column.
LC-MS/MS Analysis of Bile Acids using Ascentis® Express C18
Isobaric separation of bile acids and conjugates by LC-MS/MS on Ascentis® Express C18 column with excellent resolution and linearity.
HPLC Analysis of Fenofibrate and Its Impurities by Following the USP43-NF38 Guidelines Using a Purospher® STAR RP18e Column
An application note concerning the HPLC analysis of fenofibrate and its impurities by following the USP43-NF38 Guidelines using a Purospher® STAR RP18e Column.
Products for PFAS Analysis by Method
This page provides tables that list the products used in promulgated methods for Per- and polyfluoroalkyl substances (PFAS) analysis, organized by method and step for easy reference. As new methods are released, this page will be updated.
Desalting and Buffer Exchange for Affinity Chromatography of Antibodies
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
LRA (Lipid Removal Agent) for Biopharmaceutical Production
Lipids are naturally-occurring hydrocarbons that are poorly soluble in water and soluble in non-polar solvents such as ether and chloroform.
UHPLC Analysis of Ephedrine and Related Compounds on Supel™ Carbon LC Column
Ultra-high performance liquid chromatographic (UHPLC) separation of ephedrine and its three structural analogs based on polar retention effect on graphite (PREG) mechanism using Supel™ Carbon LC column.
UHPLC Analysis of Vitamin D2 & D3 Metabolites and Epimers on Supel™ Carbon LC Columns
Ultra high-performance liquid chromatographic (UHPLC) separation of Vitamin D Metabolites― 3-epi-25-hydroxyvitamin D2 and 25-hydroxyvitamin D2, using Supel™ Carbon LC column with baseline separation, excellent peak shape, and sensitivity.
Lewatit® Ion Exchange Resins – All-around Solutions for Chemical Processes
Chemical processes comprise the steps of processing chemical starting materials, synthesizing the products, isolating the generated materials from the reaction mixture and subsequent purification.
Selection of Purification Equipment
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
Non-volatile and Volatile Buffer Systems
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
Viral Clearance Using Capto™ Adhere
Description of viral clearance using Capto adhere from Cytiva.
Setting Column Pressure Limits for Size Exclusion Chromatography
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
Purification or Removal of Viruses including Adeno-associated Virus
This page shows how to purify or remove viruses with a Capto DeVirS, AVB Sepharose High Performance from Cytiva.
Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
Polishing of MAbs Using Capto Adhere ImpRes in Bind/Elute Mode
In these studies, the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute mode was evaluated.
Determination of Chlorogenic acid and Baicalin in compounded Lonucerae Japonicae Flos with Purospher® STAR RP-18e
Extraction and analysis of chlorogenic acid and baicalin active ingredients in traditional Chinese medicine (TCM) according to Chinese pharmacopeia monograph by HPLC with Purospher® RP-18e column
Maintenance of Multimodal Chromatography Media and Storage Conditions
This page describes the maintenance of media and storage conditions for multimodal chromatography using Cytiva products.
Protein Affinity Chromatography
Affinity chromatography is the process of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. This process allows for the highly specific and efficient purification of many diverse proteins and other compounds. The process requires
Performing a Purity and Homogeneity Check
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Protein G and Protein A Bind to Different IgG
This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.
Purification Using Protein A-based Chromatography Media
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
Characteristics of Dextrin Sepharose® High Performance Products
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
Capto Adhere Anion Exchanger for MAbs & Bind/Elute
Capto adhere from Cytiva is a multimodal strong anion exchanger for BioProcess applications.
Determination Paeonol Morroniside Loganin Liuwei Dihuang Oral Solution
Method development for the extraction and analysis of paeonol, morroniside, loganin active ingredients in traditional Chinese medicine (TCM) according to Chinese pharmacopeia monograph using HPLC-UV
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