Mammalian cell culture
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Applications:Mammalian cell culture
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General Adherent Cells
Protocol for creating create stable cell lines from both dividing and non-dividing cells for use with a lentiviral delivery system.
Platelet Derived Growth Factors (PDGF)
Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.
Lipid-induced Insulin Resistance
Lipid Induced Insulin Resistance
NF-κB and Inflammation
Chronic inflammation is an underlying factor in the development and progression of many of the chronic diseases of aging, such as arthritis, atherosclerosis, diabetes, and cancer.
Cell Culture FAQs: Bacterial Endotoxin Contamination
What is endotoxin? Frequently asked questions about bacterial endotoxin contamination of in vitro cell cultures. Details about how to endotoxin test using the LAL assay, common sources of laboratory endotoxin contamination and tips on how to avoid endotoxin contamination when
Poor Cell Growth Troubleshooting
Ensuring adequate cell growth is a critical part of collecting accurate data with cell cultures. Cells can be cultured in suspension, or as a monolayer that attaches to cultureware, such as a flask, dish or multiwell plate.
Alternative High-Yield Cell Culture Systems
Solutions for high-yield cell culture scale-up applications including multi-layered flasks (HyperFlasks/CellStacks), roller bottles, spinner and shaker flasks. Free ECACC handbook download.
Identification of Cell Culture Media with Raman Spectroscopy
Identification of cell culture media samples by traditional LC methods, such as amino acid or vitamin analysis, has high costs, and requires significant analytical expertise and laboratory space.
Hematopoietic Cytokines
Read article on hematopoietic cytokines and hematopoiesis
Applications of Percoll in Other Cell Types
The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.
Cell Clumping Troubleshooting
Common Cell Culture Problems: Cell Clumping -- Cells in suspension may attach to one another and form clumps for a variety of reasons. The most common cause of cell clumping is the presence of free DNA and cell debris in
Fibronectin
Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.
Linoleic acid in Cell Culture
Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
How to Fractionate and Analyze Gradients of Percoll
Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads
In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications
Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.
Cell Freezing and Banking
Cell Freezing and Banking
Transferrin in Cell Culture
How transferrin and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of transferrin.
Cultivating Consistency: Tips and Tricks for Successful Cell Cultures
Properly optimizing workflows and utilizing the latest best practices are the keys to obtaining consistent results and advancing research.
Oxidative Stress in Cell Culture
Read how oxygen, oxygen free radicals, oxidative stress and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing of monoclonal antibodies
Cell Death Troubleshooting in Cell Culture
Here we discuss causes of cell death, and how to assess and prevent these causes.
Good Cell Banking Practices
Overview of good cell banking practices for cell line cryopreservation purposes.
Cell Culture Protocol 5: Subculture of Suspension Cell Lines
Cell culture protocol for passaging and splitting suspension cell lines
Cell Growth to Confluence
Monitor cell culture daily to assess the morphology and density of the cell. Watch video to know the concepts of cell growth and confluence.
Nutrient Mixture F-12 Ham Formulation
Nutrient Mixture F-12 Ham Formulation
Stearic Acid in Cell Culture
Fatty acids of the n-3, n-6 and n-9 families are important supplements for cell culture systems. They are important in cell culture systems used to biomanufacture heterologous proteins, such as monoclonal antibodies.
Mammary Epithelial Cell Growth Medium
Serum-free Mammary Epithelial Cell Growth Media optimized for the in vitro cultivation of epithelial cells from the mammary gland. Protocols for handling, cell passaging, media, and product use.
Hepatocyte Growth Factor (HGF)
Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.
Palmitic Acid in Cell Culture
How palmitic acid, other fatty acids and other cell culture components
affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.
Oleic Acid in Cell Culture
How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.
Why Use Antibiotics in Cell Culture?
Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.
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