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Oligonucleotide Melting Temperature
Calculate the melting temperature (Tm) of oligonucleotides using theoretical method like the nearest neighbor or the basic method. The DNA Tm is the temperature at which 50% of the oligonucleotide is duplexed with its perfect complement and 50% is free
Dextran Sulfate
Dextran sulfates are supplied as the sodium salt forms, making them soluble and stable in water. Dextran sulfate contains approximately 17% sulfur which is equivalent to approximately 2.3 sulfate groups per glucosyl residue.
DIG RNA Labeling Kit (SP6/T7) Protocol & Troubleshooting
Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).
NBT Protocol & Troubleshooting
There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF and Nuclear Fast Red.
Random Primed DNA Labeling Kit Protocol & Troubleshooting
Random Primed DNA Labeling Kit Protocol & Troubleshooting
NBT/BCIP Stock Solution Protocol & Troubleshooting
NBT/BCIP Stock Solution Protocol & Troubleshooting
ABI3900 Synthesizer Columns with CUTAG CPG Frits
Next generation CPG Frit support for reliable, cost-effective oligo synthesis from Proligo®, for ABI 3900 and MerMade synthesizers
NBT Protocol
NBT Protocol
DIG Easy Hyb™ Granules Protocol & Troubleshooting
DIG Easy Hyb™ Granules Protocol & Troubleshooting
DIG Northern Starter Kit Protocol & Troubleshooting
In general, it is possible to use DNA probes for northern blots (PCR or random-primed DNA probes). It is however important to keep in mind that DNA probes are best detecting abundant messages, such as housekeeping genes.
DIG RNA Labeling Mix Protocol Troubleshooting
Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).
NBT/BCIP Ready-to-Use Tablets Protocol Troubleshooting
NBT/BCIP Ready-to-Use Tablets Protocol Troubleshooting
Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting
In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.
PTK/PTP Expression Profiling
PTK/PTP Expression Profiling
Northern and Southern Blot Protocols & Introduction
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.