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FastStart™ Taq DNA Polymerase, dNTPack Protocol & Troubleshooting
The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.
KAPA3G Plant PCR Kits FAQs
Frequently asked questions (FAQs) for KAPA3G Plant PCR Kits.
Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)
Preserved samples from medical, forensic, museum and other archival collections represent a rich source of study material, much of it meticulously collected, characterized and preserved through many decades of work by experts in the field.
Quantitative PCR and Digital PCR Detection Methods
Fluorescent dyes or probes are included in PCR mixes to monitor the change in NA amplicon concentration as the reaction proceeds.
Whole Genome Amplification for Single Cell Biology
Whole genome amplification (WGA) offers a means to overcome the above restrictions for single-cell genomic analyses. WGA has been described as a non-specific amplification technique that affords an amplified product completely representative of the initial starting material.
PCR Troubleshooting Tips
When developing a PCR troubleshooting protocol, it is important to be open to any possible sources of error, however insignificant they may seem, in order to explore each potential problem independently.
Oligonucleotide Quality Control by Mass Spectrometry
Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.
Working with PCR
General Considerations; Factors to Consider in RT-PCR; Prevention of Carryover Contamination; Troubleshooting
Extract-N-Amp™ Tissue Feature Article
The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the
ThermaGenix Product Overview
How to stop nonspecific bands and off target primers in PCR.
FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting
The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.
RT-PCR / RT-qPCR Troubleshooting
Developing a PCR or RT-PCR/RT-qPCR troubleshooting protocol so that data are reliable is essential. Potential sources of RT-PCR or PCR error and problems include operator error, the PCR master mix, and oligo design. This PCR troubleshooting guide outlines and details
Polymerase Chain Reaction
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
Whole Transcriptome Amplification of RNA from Low Cell-Number Samples
The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.
Oligonucleotide Array CGH Analysis of a Robust Whole Genome Amplification Method
In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, somewhat hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome
Extract-N-Amp™ Blood PCR Kit Protocol
The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.
KAPA Express Extract Kit FAQs
Frequently asked questions (FAQs) for KAPA Express Extract Kit.
KAPA2G Robust PCR Kits FAQs
Frequently asked questions (FAQs) for KAPA2G Robust PCR Kits.
A highly efficient method to concentrate DNA for forensic STR genotyping using DNAstable®
Forensic laboratories routinely use STR genotyping for identity testing of biological samples. However, forensic samples often contain low copy numbers of target DNA, making it difficult to obtain complete STR profiles.
Modification of the WTA2 Amplification Product for Next Generation Sequencing
Transplex Whole Transcriptome Amplification (WTA2) exponentially amplifies RNA producing a double-stranded cDNA library while precisely maintaining differential levels of individual transcripts in test and reference samples.
Troubleshooting PCR and RT-PCR Amplification
This page shows PCR and RT-PCR amplification troubleshooting.
Dual-Labeled Probes in Digital PCR
Digital PCR (dPCR) is an end-point PCR method that is used for absolute quantification and for analysis of minority sequences against a background of similar majority sequences.
MIQE – Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Our qPCR services, including primer and probe designs, assay protocol development, troubleshooting, and data analysis support, adhere to MIQE, which allows you to publish or bring your product to market faster and with confidence.
What is Hot Start PCR?
Learn about the benefits of using Hot Start PCR reagents to suppress enzymatic activity before PCR reaction tubes enter the thermal cycler.
Whole Genome Amplification for Single Cell Biology
The ultimate biological unit lies within a single cell. Many biological disciplines have taken aim to elucidate the causes of cellular differentiation at this level.
OligoArchitect™ Assay Design
Efficient qPCR relies on good assay design. Since the invention of PCR, many parameters have been identified as important for assay quality, such as estimates of oligo temperature characteristics, GC content and folding properties.
Long and Accurate PCR
Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.
Optimizing Crude Sample Plant PCR
Overcoming potent inhibitors and sample diversity in direct plant PCR.
Introduction and Historical Timelines
Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.
Using PCR to Detect Viral Agents in Animal Sera
The PCR method for detection of viral agents in FBS and other animal sera can help to ensure virus-free serum for cell culture.
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