Primary cell culture
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Applications:Primary cell culture
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Cell Viability and Proliferation XTT Assay Protocol Guide
Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.
In Vitro Expansion of CD34+/CD38- Human Hematopoietic Progenitor Cells in a Serum-free and Xeno-free Stem Cell Culture Media
In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products
Protocol for Preparation and Cultivation of PromoCell® Cryopreserved and Proliferating Cells
Protocol for the seeding and cultivation of primary cells received in cryopreserved or proliferating formats. Information on primary cell culture, cell handling and passaging.
In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells
Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.
Trilineage Differentiation of Multipotent Human Mesenchymal Stem Cells (MSCs) into Osteocytes, Adipocytes and Chondrocytes
Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.
Ficoll® 400 for Gradient Centrifugation
Ficoll 400® is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400® is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400® is very hydrophilic and extremely water-soluble.
Primary Cell Culture FAQ
Primary Cell Culture: Frequently Asked Questions (FAQs)
Human Dermal Fibroblasts (HDF) Culture Protocol
This protocol contains: Storage - Preparation for Culturing - Culturing HDF - Subculturing HDF
Human Astrocytes (HA) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Epidermal Keratinocytes (HEK) Culture Protocol
Storage - Preparation for Culturing - Culturing HEK - Subculturing HEK
Human Mononuclear Cells – Periphery Blood (HMNC-PB) Culture Protocol
Technical information for working with human mononuclear cells – periphery blood including thawing, subculturing and cryopreservation
Human Osteoblasts (HOb) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Bovine Aortic Endothelial Cells (BAOEC) Culture Protocol
Technical information for working with bovine aortic endothelial cells including thawing, subculturing and cryopreservation.
Canine Aortic Endothelial Cells (CnAOEC) Culture Protocol
Technical information for working with Canine Aortic Endothelial Cells including thawing, subculturing and cryopreservation
Bovine Aortic Smooth Muscle Cells (BAOSMC) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Pulmonary Artery Endothelial Cells (HPAEC) Culture Protocol
Technical information for working with human pulmonary artery endothelial cells including thawing, subculturing and cryopreservation
Human Coronary Artery Endothelial Cells (HCAEC) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Pulmonary Artery Smooth Muscle Cells (HPASMC) Culture Protocol
Technical information for working with human pulmonary artery smooth muscle cells including thawing, subculturing and cryopreservation
Human Epidermal Melanocytes (HEM) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Preadipocytes (HPAd) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Fibroblast-Like Synoviocytes (HFLS) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Lung Microvascular Endothelial Cells (HLMVEC) Culture Protocol
Technical information for working with human lung microvascular endothelial cells including thawing, subculturing and cryopreservation
Promocell
Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.
Human Aortic Endothelial Cells (HAOEC) Culture Protocol
Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.
Human Mesenchymal Stem Cells and Growth/Differentiation Media
Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and
ADCC Protocol using BioIVT Cryopreserved NK Effector Cells
Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.
ECM Coating Protocols for Millicell®
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
Culturing Human Aortic Smooth Muscle Cells
Technical information for working with human aortic smooth muscle cells including thawing, subculturing and cryopreservation.
In Vitro Differentiation of Human PBMC Derived Monocytes into M1 or M2 Macrophages in a Serum-free and Xeno-free Cell Culture Media
Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.
Human Bronchial Epithelial Cell line
Technical information for working with human bronchial epithelial cell line including thawing, subculturing and cryopreservation.
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