Stem cell culture

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Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

Robust Differentiation of Human iPSCs into Lineage-Specific Neurons and Glial Cells Utilizing Dual-SMAD Inhibition

Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.

Hematopoietic Cytokines

Read article on hematopoietic cytokines and hematopoiesis

3D Pluripotent Stem Cells: Simplified Culture and Realistic Differentiation

This white paper illustrates the importance of three-dimensional (3D) cell culture in the production of pluripotent stem cell (PSC)-derived models of embryo development and differentiation. It contains a general overview of embryoid bodies (EBs) and a comparison between the various

Single Transfection of a Synthetic Polycistronic Self-replicative RNA Yields High Numbers of Transgene-free Human iPSCs

The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

Mesenchymal Stem Cell Culture

Highly characterized human mesenchymal stem cell lines from bone-marrow, adipose and umbilical cord along with serum-free MSC expansion and differentiation media.

Pluripotent and Multipotent Stem Cells

Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling

Mesenchymal Stem Cell FAQs

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Growth Factors in Stem Cell Biology

Role of growth factors in stem cell differentiation and various growth factors for your research at sigmaaldrich.com

Neural Stem Cell FAQs

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

Hematopoietic Stem & Immune Cell Culture

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

Naive Pluripotent Stem Cell Culture in Serum-Free Media

Naive pluripotent stem cells cultured in vitro using specialized media and inhibitors mimic "ground-state" cells from blastocysts.

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

Mouse Embryonic Fibroblasts (MEF Feeder Cells)

Mouse embryonic fibroblasts (MEFs) serve as a feeder layer for both mouse and human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPSCs).

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

IPS Cells or Induced Pluripotent Stem Cell FAQs

FAQs and information about IPS Cells or induced pluripotent stem cells (iPSCs). Including what are IPS cells, learning stem cell basics, reprogramming methods, and iPS cell culture techniques.

ReNcell® Human Neural Progenitors: Renewable and Consistent Human Neural Stem Cell Lines that Differentiate into Functional Neurons

ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.

Neuronal Mesenchymal Stem Cells

Protocol to differentiate human mesenchymal stem cells into neurons with extensive dendrites and axons in 3 days. Explore over 350 PromoCell products on Sigma.com.

ECMatrix™ Laminin Substrates are Superior Xeno-free Extracellular Matrix Coatings for Feeder-free Human Pluripotent Stem Cell Cultures

No precoat needed. Single cell iPSC passaging. Human recombinant laminin-511 E8 allows greater iPS cell adhesion and proliferation rates vs. Matrigel and laminin-521.

Canonical Wnt / β-Catenin Signaling

We review the Wnt signaling pathway (also, canonical Wnt or beta (β)-catenin pathway) and provide links to related products at sigmaaldrich.com.

Cancer Stem Cell Detection with AldeRed™ 588-A

AldeRed™ 588-A detects ALDH activity in cancer stem cells, aiding in cancer research.

HumanKine® Thermostable bFGF

Fibroblast growth factors (FGFs) regulate developmental pathways and mesoderm/ectoderm patterning in early embryonic development.

Induced Pluripotent Stem Cell Differentiation Protocols

We offer answers to frequently asked questions and a large collection of cell culture media, supplements, and bioactive small molecules used to control the cell fate of human iPSCs.

A Serum-Free and Feeder-Free Defined Medium That Enables Weekend-Free Culture of Undifferentiated Human ES/iPS Cells

Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.