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Transdermal Diffusion Testing with Strat-M^® Synthetic Skin Membranes using Franz Diffusion Cell Systems

Step-by-step protocol and video for transdermal diffusion testing using Strat-M® synthetic skin membranes.

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

Cell Viability and Proliferation XTT Assay Protocol Guide

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

Analytical Method: Determination of the Solution Color of Raw Sugars, Brown Sugars and Colored Syrups at pH 7.0 (According to ICUMSA Method GS1/3-7)

Method: Spectrophotometric Color Determination at 420 nm Zero adjustment For each measurement series, a zero adjustment is required. It is recommended to use the same cell for zero adjustment and for sample measurement. The zeroing procedure for the measurement series is automatically prompted by

DNase I Recombinant, RNase-free Protocol

DNase I from bovine pancreas is a glycoprotein of Mr 37000. A special procedure is used to remove RNases from the DNase preparation.

Webinar: High-precision PCR using ThermaGenix Reagents

Standard PCR Protocol

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

Multiplex Real-Time PCR

Multiplex qPCR employing probe-based chemistries is a demanding application that often requires extensive optimization and validation.

TeloTAGGG Telomerase PCR ELISA Protocol & Troubleshooting

The TeloTAGGG Telomerase PCR ELISA is used for sensitive qualitative detection of telomerase activity.

Rapid SYBR® Green qPCR on the Illumina Eco™ Real-Time PCR System

Fast real-time PCR is a demanding application that requires consistent and reproducible results from the difficult amplicons and low-volume reactions

Spermine Tetrahydrochloride for Molecular Biology

This product is suitable for DNA precipitation from low salt aqueous buffers. It has been tested for the absence of proteases and nucleases.

Genomic DNA Purification from Sample on FTA Elute

This page shows genomic DNA purification from sample on FTA elute from Cytiva.

DNAgard® Blood Protocol

DNAgard Blood is designed for shipping and storage of DNA in mammalian whole blood. DNA in blood is protected by the unique stabilization properties of DNAgard Blood

Processing Small Tissue or Cell Samples using Sample Grinding Kit

Sample Grinding Kit from Cytiva is designed to disrupt cell or tissue samples for protein extraction.

Immunoprecipitation Kit (Protein G) Protocol & Troubleshooting

Coupling Small Ligands Through Carboxyl Groups via a Spacer Arm

Ligands are coupled in a simple one-step procedure in the presence of a coupling reagent, carbodiimide. The carbodiimides may be regarded as anhydrides of urea.

Using Duolink® in Protein Interaction Studies

The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.

Protein Determination by the Bicinchoninic Acid (BCA) Method

A standard procedure for the quantitative determination of total protein concentration of a solution by the BCA method.

Performing a Separation with HiTrap® IgM Purifcation HP

How to perform a separation with the HiTrap® IgM Purifcation HP from Cytiva which is an Affinity Chromatography product optimized for purification of monoclonal IgM from hybridoma cell culture.

cOmplete™, EDTA-free Protocol

It is possible dissolve 1 tablet in 2 mL double dist. water (results in a 25x stock solution) without any problems.

Puriﬁcation using StrepTactin Sepharose® High Performance

This page shows how to purify Strep-tag II recombinant proteins using StrepTactin Sepharose® High Performance from Cytiva.

Ion Channel Flux Assays and Protocols

A review and resources for ion channel flux assays and protocols, along with liposome formation and proteoliposome preparation protocols

Duolink® PLA Multicolor Detection Protocol

Duolink® PLA Probemaker Guide

Guide to create custom PLA probes for immunofluorescent or brightfield detection.

Coating Tissue Culture Plates with Vitronectin

Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. This protocol is for coating a 96-well plate and other surfaces.

Analytical Method: Selenium in Water

Method: Photometric determination with 3,3'-Diaminobenzidine

User Guide Dispensing Pressure Vessel Fitting Kit

This is a User Guide Dispensing Pressure Vessel Fitting Kit from Millipore.

LC/MS Analysis of Glyphosate and Metabolites on apHera™ NH2, 2 mm I.D. Column

Fast LC-MS-MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3

We presents a robust method for analyzing 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in serum using a multiplexed LC-MS-MS system.

HPLC Analysis of Immunoglobulin G (IgG) on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution

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