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  • Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing.

Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing.

Metabolic engineering (2015-07-05)
Yifan Li, Zhenquan Lin, Can Huang, Yan Zhang, Zhiwen Wang, Ya-Jie Tang, Tao Chen, Xueming Zhao
ABSTRACT

Engineering cellular metabolism for improved production of valuable chemicals requires extensive modulation of bacterial genome to explore complex genetic spaces. Here, we report the development of a CRISPR-Cas9 based method for iterative genome editing and metabolic engineering of Escherichia coli. This system enables us to introduce various types of genomic modifications with near 100% editing efficiency and to introduce three mutations simultaneously. We also found that cells with intact mismatch repair system had reduced chance to escape CRISPR mediated cleavage and yielded increased editing efficiency. To demonstrate its potential, we used our method to integrate the β-carotene synthetic pathway into the genome and to optimize the methylerythritol-phosphate (MEP) pathway and central metabolic pathways for β-carotene overproduction. We collectively tested 33 genomic modifications and constructed more than 100 genetic variants for combinatorially exploring the metabolic landscape. Our best producer contained15 targeted mutations and produced 2.0 g/L β-carotene in fed-batch fermentation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Cas9 Lambda Red Homologous Recombination Plasmid for E. Coli
Sigma-Aldrich
CRISPR LacZ Positive Control plasmid for Bacteria
Sigma-Aldrich
CRISPR Non-Target Negative Control Plasmid for Bacteria