Merck
  • Home
  • Search Results
  • Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.

Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.

Nature communications (2020-09-25)
Jan Krivanek, Ruslan A Soldatov, Maria Eleni Kastriti, Tatiana Chontorotzea, Anna Nele Herdina, Julian Petersen, Bara Szarowska, Marie Landova, Veronika Kovar Matejova, Lydie Izakovicova Holla, Ulrike Kuchler, Ivana Vidovic Zdrilic, Anushree Vijaykumar, Anamaria Balic, Pauline Marangoni, Ophir D Klein, Vitor C M Neves, Val Yianni, Paul T Sharpe, Tibor Harkany, Brian D Metscher, Marc Bajénoff, Mina Mina, Kaj Fried, Peter V Kharchenko, Igor Adameyko
ABSTRACT

Understanding cell types and mechanisms of dental growth is essential for reconstruction and engineering of teeth. Therefore, we investigated cellular composition of growing and non-growing mouse and human teeth. As a result, we report an unappreciated cellular complexity of the continuously-growing mouse incisor, which suggests a coherent model of cell dynamics enabling unarrested growth. This model relies on spatially-restricted stem, progenitor and differentiated populations in the epithelial and mesenchymal compartments underlying the coordinated expansion of two major branches of pulpal cells and diverse epithelial subtypes. Further comparisons of human and mouse teeth yield both parallelisms and differences in tissue heterogeneity and highlight the specifics behind growing and non-growing modes. Despite being similar at a coarse level, mouse and human teeth reveal molecular differences and species-specific cell subtypes suggesting possible evolutionary divergence. Overall, here we provide an atlas of human and mouse teeth with a focus on growth and differentiation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Fluoromount Aqueous Mounting Medium, for use with fluorescent dye-stained tissues
Sigma-Aldrich
TWEEN® 20, for molecular biology, viscous liquid
Sigma-Aldrich
Anti-NOTUM antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-CLDN10 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
DAPI, for nucleic acid staining
Sigma-Aldrich
Hanks′ Balanced Salt solution, Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Anti-SOX9 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution