• Home
  • Search Results
  • Determination of log D via automated microfluidic liquid-liquid extraction.

Determination of log D via automated microfluidic liquid-liquid extraction.

Journal of medicinal chemistry (2008-08-01)
Muhammad Alimuddin, Daniel Grant, Daryl Bulloch, Noelle Lee, Martin Peacock, Russell Dahl
ABSTRACT

A method for log D (pH 7.4) measurement was developed using microfluidic liquid-liquid extraction. Values were determined for 26 compounds and compared to results obtained via shake-flask methods. Excellent correlation between the values obtained via both methods was achieved (R(2) = 0.994). The developed methodology is amenable to automation, enabling high-throughput determination of large compound collections.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Caffeine, powder, ReagentPlus®
Sigma-Aldrich
Hydrocortisone, BioReagent, suitable for cell culture
Sigma-Aldrich
Hydrocortisone solution, 50 μM, sterile-filtered, BioXtra, suitable for cell culture
Sigma-Aldrich
Acetaminophen, BioXtra, ≥99.0%
Sigma-Aldrich
Hydrocortisone, ≥98% (HPLC)
Sigma-Aldrich
Caffeine, anhydrous, 99%, FCC, FG
Sigma-Aldrich
Trimethoprim, ≥98% (HPLC)
Sigma-Aldrich
Hydrocortisone, γ-irradiated, powder, BioXtra, suitable for cell culture
Sigma-Aldrich
Acetaminophen, meets USP testing specifications, 98.0-102.0%, powder
Sigma-Aldrich
Acetaminophen, analytical standard
Sigma-Aldrich
Caffeine, Sigma Reference Standard, vial of 250 mg
Supelco
Melting point standard 235-237°C, analytical standard
Sigma-Aldrich
Hydrocortisone-Water Soluble, BioReagent, suitable for cell culture
Sigma-Aldrich
Atenolol, ≥98% (TLC), powder
Supelco
Mettler-Toledo Calibration substance ME 18872, Caffeine, analytical standard, for the calibration of the thermosystem 900, traceable to primary standards (LGC)
Sigma-Aldrich
Trimethoprim, crystallized, ≥99.0% (HPLC)
Sigma-Aldrich
Caffeine, meets USP testing specifications, anhydrous
Supelco
Trimethoprim, VETRANAL®, analytical standard
Supelco
Caffeine solution, analytical standard, 1.0 mg/mL in methanol
Sigma-Aldrich
Caffeine, anhydrous, tested according to Ph. Eur.
Sigma-Aldrich
3-Chlorophenol, 98%
Sigma-Aldrich
Hydrocortisone 21-acetate, ≥98% (HPLC)
Sigma-Aldrich
Caffeine, BioXtra
Sigma-Aldrich
Hydrocortisone, meets USP testing specifications
Sigma-Aldrich
Hydrocortisone 21-acetate, meets USP testing specifications
Supelco
Hydrocortisone, VETRANAL®, analytical standard