Merck
  • Home
  • Search Results
  • Bioinspired Dopamine/Mucin Coatings Provide Lubricity, Wear Protection, and Cell-Repellent Properties for Medical Applications.

Bioinspired Dopamine/Mucin Coatings Provide Lubricity, Wear Protection, and Cell-Repellent Properties for Medical Applications.

Advanced healthcare materials (2020-09-18)
Jian Song, Theresa M Lutz, Nora Lang, Oliver Lieleg
ABSTRACT

Even though medical devices have improved a lot over the past decades, there are still issues regarding their anti-biofouling properties and tribological performance, and both aspects contribute to the short- and long-term failure of these devices. Coating these devices with a biocompatible layer that reduces friction, wear, and biofouling at the same time would be a promising strategy to address these issues. Inspired by the adhesion mechanism employed by mussels, here, dopamine is made use of to immobilize lubricious mucin macromolecules onto both manufactured commercial materials and real medical devices. It is shown that purified mucins successfully adsorb onto a dopamine pre-coated substrate, and that this double-layer is stable toward mechanical challenges and storage in aqueous solutions. Moreover, the results indicate that the dopamine/mucin double-layer decreases friction (especially in the boundary lubrication regime), reduces wear damage, and provides anti-biofouling properties. The results obtained in this study show that such dopamine/mucin double-layer coatings can be powerful candidates for improving the surface properties of medical devices such as catheters, stents, and blood vessel substitutes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Dopamine hydrochloride
Sigma-Aldrich
Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Greiner CELLSTAR® 96 well plates, flat bottom clear wells
Sigma-Aldrich
MEM Non-essential Amino Acid Solution (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
Minimum Essential Medium Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Ethidium homodimer, suitable for fluorescence, ~90% (HPCE)
Sigma-Aldrich
Pentaethylene glycol monododecyl ether, BioXtra, ≥98.0% (GC)