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Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening.

STAR protocols (2020-10-13)
Huyen Thi Lam Nguyen, Alice Soragni
ABSTRACT

Tumor organoids are promising tools for cancer biology investigations and preclinical drug screenings because they are often representative of the histology and drug responses of patients. Here, we introduce a facile protocol to overcome technical limitations by generating patient-derived tumor organoids using a simplified ring-like geometry. This facilitates media exchange and drug treatment for histopathology characterization and automated high-throughput drug screenings. For complete details on the use and execution of this protocol, please refer to Phan et al. (2019).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
CryoStor® cell cryopreservation media, CS10