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Cholesterol exchange between human serum isolated lipoproteins. Effect of protein concentration.

Biomedica biochimica acta (1991-01-01)
E Velazquez, A Montes, J M Ruiz-Albusac
ABSTRACT

The in vitro transfer of radiolabelled unesterified cholesterol from human low- and very low- to high-density lipoproteins in the presence of either lipoprotein deficient serum or bovine serum albumin has been studied. The rate of transfer was faster from LDL (t1/2 = 44 min) than from VLDL (55 min). The presence of 2% protein had no effect on the transfer. However, 10% albumin or lipoprotein deficient serum reduced t1/2 by 35%, which indicates no specific effect of a plasma protein on the rate of transfer of unesterified cholesterol.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Lipoprotein Deficient Serum from human plasma, sterile-filtered, frozen liquid