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  • Discrete cytosolic macromolecular BRAF complexes exhibit distinct activities and composition.

Discrete cytosolic macromolecular BRAF complexes exhibit distinct activities and composition.

The EMBO journal (2017-01-18)
Britta Diedrich, Kristoffer Tg Rigbolt, Michael Röring, Ricarda Herr, Stephanie Kaeser-Pebernard, Christine Gretzmeier, Robert F Murphy, Tilman Brummer, Jörn Dengjel
ABSTRACT

As a central element within the RAS/ERK pathway, the serine/threonine kinase BRAF plays a key role in development and homeostasis and represents the most frequently mutated kinase in tumors. Consequently, it has emerged as an important therapeutic target in various malignancies. Nevertheless, the BRAF activation cycle still raises many mechanistic questions as illustrated by the paradoxical action and side effects of RAF inhibitors. By applying SEC-PCP-SILAC, we analyzed protein-protein interactions of hyperactive BRAF

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Lys-Lys-Lys-Lys, ≥95% (TLC)
Sigma-Aldrich
17-(Allylamino)-17-demethoxygeldanamycin, ≥98% (HPLC), solid