Merck

Neurofilaments bind tubulin and modulate its polymerization.

The Journal of neuroscience : the official journal of the Society for Neuroscience (2009-09-04)
Arnaud Bocquet, Raphael Berges, Ronald Frank, Patrick Robert, Alan C Peterson, Joël Eyer
ABSTRACT

Neurofilaments assemble from three intermediate-filament proteins, contribute to the radial growth of axons, and are exceptionally stable. Microtubules are dynamic structures that assemble from tubulin dimers to support intracellular transport of molecules and organelles. We show here that neurofilaments, and other intermediate-filament proteins, contain motifs in their N-terminal domains that bind unassembled tubulin. Peptides containing such motifs inhibit the in vitro polymerization of microtubules and can be taken up by cultured cells in which they disrupt microtubules leading to altered cell shapes and an arrest of division. In transgenic mice in which neurofilaments are withheld from the axonal compartment, axonal tubulin accumulation is normal but microtubules assemble in excessive numbers. These observations suggest a model in which axonal neurofilaments modulate local microtubule assembly. This capacity also suggests novel mechanisms through which inherited or acquired disruptions in intermediate filaments might contribute to pathogenesis in multiple conditions.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-Neurofilament 160 antibody produced in mouse, clone NN18, ascites fluid
Sigma-Aldrich
Anti-Neurofilament 200 antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
Sigma-Aldrich
Anti-Neurofilament 200 antibody, Mouse monoclonal, clone NE14, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-β-Tubulin antibody produced in mouse, clone TUB 2.1, ascites fluid
Sigma-Aldrich
Monoclonal Anti-Neurofilament 200 antibody produced in mouse, clone NE14, ascites fluid
Sigma-Aldrich
Monoclonal Anti-α-Tubulin antibody produced in mouse, clone DM1A, ascites fluid
Sigma-Aldrich
Monoclonal Anti-β-Tubulin III antibody produced in mouse, clone SDL.3D10, ascites fluid
Sigma-Aldrich
Monoclonal Anti-Neurofilament 68 antibody produced in mouse, clone NR4, ascites fluid
Sigma-Aldrich
Anti- Neurofilament 200-FITC antibody, Mouse monoclonal, clone NE14, purified from hybridoma cell culture