In SPME, you can adsorb analytes from a liquid sample, by immersion or headspace extraction, or a solid sample, by headspace extraction, using a polymer-coated fused silica fiber. Analytes are desorbed from the fiber by exposing the fiber in the injection port of a GC or in the desorption chamber of an SPME/ HPLC interface.
Determine the type of fiber you need according to the molecular weights and polarity of the analytes.
Most of these fibers are compatible with HPLC solvents, but the 100µM and 30µM PDMS-coated fibers cannot be used with hexane. SPME fiber holders are available in two versions, one for manual use and one for use with autosamplers or with our SPME/HPLC interface. Both versions include the following features:
The manual holder has an adjustable needle gauge that controls the depth of fiber introduction into the sample vial or injection port. A spring retracts the fiber into the protective needle and a locking mechanism secures the fiber in the exposed position during extraction or desorption.
The automated holder is similar in design to the manual version. The autosampler controls fiber movement, allowing automatic sample extraction. The automated holder also is required for use with an SPME/ HPLC interface.
A specialized type of manual SPME holder, the SPME portable sampler, allows you to concentrate organics from air or water, in the field, then store them for transport to the laboratory.
Some typical applications for SPME are: