Three-dimensional cell culture is an innovative approach to modeling the morphological effects of oncogenesis and development using three-dimensional microenvironments. Extracellular matrix proteins form hydrogels under physiological conditions that mimic the cell environment in vivo, and as a result, these cells assume structural and functional characteristics of their emanating tissues. The 3D Culture Matrix BME Coated 96 Well Plate (Product No. 3445-096-CP) is coated with a reconstituted basement membrane matrix to provide the environment that is common for epithelial or endothelial cell types, and this plate provides a convenient, standardized, physiologically predictive format for evaluating the pharmacological effects of compounds for these cell culture models.
Product is stable for a minimum of 3 months from date of shipment when stored at –20 °C in a manual defrost freezer. For optimal stability, store at –80 °C. Avoid freeze-thaw cycles.
This procedure must be conducted in an aseptic environment, such as a laminar flow hood or clean room, using aseptic technique to prevent contamination.
Recommendations for analysis:
Cell number may be determined using 3D Culture Proliferation Core Kit (Product No. 3445-096-CK).
Figure 1.Three-Dimensional Cellular Structures. Staining of MCF-10A cells after sixteen days is 3-D Culture Matrix™ RGF BME with: A) Cell Staining Kit (structural), B) SYBR® Green (nuclear), and C) MitoShift™ (mitochondrial potential); and staining of PC-3 cells after twelve days in3-D Culture Matrix™ RGF BME with: D) Calcein AM (cell viability), E) CPA dye 1 (nuclear) and F) Depsipher™ (mitochondrial potential)
3-D Culture Matrix is a trademark of Trevigen, Inc.
Cultrex is a registered trademark of Trevigen, Inc.
Note: This product is made and marketed under patent license from the United States Public Health Service. Ref. U.S. Patent 4,829,000 issued May 9, 1989 and U.S. Patent 5,158,874 issued October 27,1992, all entitled Reconstituted Membrane Complex with Biological Activity.