Induced Pluripotent Stem Cell Differentiation Protocols

Adult somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) with the overexpression of key reprogramming genes (OCT4, KLF4, SOX2, cMYC, NANOG and LIN28). Human iPSCs have the unique ability to differentiate into any cell type of the body including:

  • Ectodermal: Neuron, Astrocyte, Oligodendrocyte, Retinal Epithelial Cell (RPE), Epidermal, Hair and Keratinocytes.
  • Endodermal: Hepatocyte, Pancreatic β-islet Cell, Intestinal Epithelial Cell, Lung Alveolar Cells.
  • Mesodermal: Hematopoietic, Endothelial Cell, Cardiomyocyte, Smooth Muscle Cell, Skeletal Muscle Cell, Renal cell, Adipocyte, Chondrocyte and Osteocytes.
IPSC Pathway

Figure 1.IPSC Pathway

We offer a large collection of cell culture media, supplements, bioactive small molecules, and growth factors used to control the cell fate of human iPSCs. The table below highlights the most widely used protocols, media and characterization antibodies used to differentiate human iPSC’s into different cell lineages.