Merck
All Photos(1)

40718

Sigma-Aldrich

Diethyl pyrocarbonate

96% (NT)

Synonym(s):
Diethyl dicarbonate, DEPC, Diethyl oxydiformate, DEP, Ethoxyformic acid anhydride
Linear Formula:
O(COOC2H5)2
CAS Number:
Molecular Weight:
162.14
Beilstein:
637031
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.22

Nivel de calidad

200

ensayo

96% (NT)

formulario

liquid

color

APHA: ≤20

índice de refracción

n20/D 1.398 (lit.)
n20/D 1.398

bp

93-94 °C/18 mmHg (lit.)

solubilidad

95% ethanol: 4.5 g/10 mL, clear, colorless

densidad

1.121 g/mL at 20 °C
1.101 g/mL at 25 °C (lit.)

enviado en

wet ice

temp. de almacenamiento

2-8°C

SMILES string

CCOC(=O)OC(=O)OCC

InChI

1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3

InChI key

FFYPMLJYZAEMQB-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Descripción general

Diethyl pyrocarbonate (DEP) is a bactericidal agent and decomposes in water to CO2 and ethanol. DEP inhibits enzymes such as DNase and RNase without affecting nucleic acids. It is useful for the preparation of mRNA from tissues.

Aplicación

Diethyl pyrocarbonate may be used in the following studies:
  • For the modification of histidyl residues in proteins.
  • As a nuclease inhibitor, for the extraction of undegraded nucleic acids from etiolated and green plant tissues.
  • For the modification of linear and supercoiled plasmid DNAs.
  • As a chemical probe to investigate the secondary structure in negatively supercoiled DNA.
  • For cabethoxylation of histidine residues of actin.
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

Envase

25, 100 mL in poly bottle

Acciones bioquímicas o fisiológicas

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Acute Tox. 4 Oral

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 3

Punto de inflamabilidad F

156.2 °F - closed cup

Punto de inflamabilidad C

69 °C - closed cup

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service