Merck
All Photos(5)

860336

Sigma-Aldrich

3,3′,5,5′-Tetrametilbencidina

≥99%

All Photos(5)
Synonym(s):
BM azul, Sure Blue TMB, Sustrato TMB, TMB, Transferencia de TMB Plus
Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS Number:
54827-17-7
Molecular Weight:
240.34
Beilstein:
2808541
EC Number:
259-364-6
MDL number:
MFCD00007748
PubChem Substance ID:
24888505

Quality Level

200

Assay

≥99%

form

powder

mp

168-171 °C (lit.)

storage temp.

2-8°C

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

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This Item
8774809743T5525
3,3′,5,5′-Tetramethylbenzidine ≥99%

Sigma-Aldrich

860336

3,3′,5,5′-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine ≥98.0% (NT)

Sigma-Aldrich

87748

3,3′,5,5′-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine standard for GC

Supelco

09743

3,3′,5,5′-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine tablet, 1 mg substrate per tablet

Sigma-Aldrich

T5525

3,3′,5,5′-Tetramethylbenzidine

assay

≥99%

assay

≥98.0% (NT)

assay

≥99.0% (GC)

assay

-

form

powder

form

powder

form

-

form

tablet

mp

168-171 °C (lit.)

mp

166-171 °C, 168-171 °C (lit.)

mp

166-171 °C, 168-171 °C (lit.)

mp

168-171 °C (lit.)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

Quality Level

200

Quality Level

300

Quality Level

100

Quality Level

200

General description

La 3,3′,5,5′-tetrametilbencidina es un colorante sensible a la peroxidasa. Es una mejor alternativa a la siringaldacina para la determinación del cloro en el agua debido a su estabilidad y la ausencia de interferencia de los iones calcio y magnesio.

Application

La 3,3′,5,5′-tetrametilbencidina (TMB) es un sustituto no carcinógeno (prueba de Ames negativa) de la bencina, adecuada como sustrato de la peroxidasa para procedimientos ELISA. Este sustrato rinde un producto final soluble que es de color azul claro y puede leerse espectrofotométricamente a 370 o 620-650 nm. La reacción de la TMB puede detenerse con H2SO4 2 M (que produce un color amarillo) y leerse a 450 nm. Reactivo sensible y específico para la detección de sangre, análisis de la hemoglobina, análisis de peroxidasa.

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Warning

Hazard Statements

H302 - H351 - H413

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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