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TMB substrate, TMB, Sure Blue TMB, BM blue, TMB Blotting Plus
Fórmula lineal:
Número de CAS:
Peso molecular:
Número de EC:
Número MDL:
ID de la sustancia en PubChem:






168-171 °C (lit.)

temp. de almacenamiento


SMILES string




InChI key


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Descripción general

3,3′,5,5′-Tetramethylbenzidine is a peroxidase sensitive dye. It is a better alternative to syringaldazine for the determination of chlorine in water due to its product stability and no interference from calcium and magnesium ions.


100 mg in glass bottle
1, 5, 25 g in glass bottle


3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Código de clase de almacenamiento

11 - Combustible Solids



Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Colorimetric method for determination of chlorine with 3, 3?, 5, 5?-tetramethylbenzidine.
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