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M6250

Sigma-Aldrich

2-Mercaptoethanol

≥99.0%

Sinónimos:
β-Mercaptoethanol, BME, Thioethylene glycol, 2-Hydroxyethylmercaptan
Fórmula lineal:
HSCH2CH2OH
Número de CAS:
Peso molecular:
78.13
Beilstein:
773648
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.22

Nivel de calidad

200

densidad de vapor

2.69 (vs air)

presión de vapor

1 mmHg ( 20 °C)

ensayo

≥99.0%

formulario

liquid

lim. expl.

18 %

concentración

14.3 M (pure liquid)

índice de refracción

n20/D 1.500 (lit.)

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

densidad

1.114 g/mL at 25 °C (lit.)

temp. de almacenamiento

2-8°C

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

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Descripción general

2-mercaptoethanol is a thiol compound, commonly used as a reducing agent in organic reactions.

Envase

10, 100, 250, 500 mL in glass bottle
1, 2.5 L in glass bottle

Aplicación

2-mercaptoethanol is widely used for retarding oxidation of biological compounds in solution.
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

Código de clase de almacenamiento

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Punto de inflamabilidad F

165.2 °F - closed cup

Punto de inflamabilidad C

74 °C - closed cup

Equipo de protección personal

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificado de Análisis

Certificado de origen

Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.
Morrissey JH.
Analytical Biochemistry, 117(2), 307-310 (1981)
Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines.
Asfari M, et al.
Endocrinology, 130(1), 167-178 (1992)
Manching Ku et al.
PLoS genetics, 4(10), e1000242-e1000242 (2008-11-01)
In embryonic stem (ES) cells, bivalent chromatin domains with overlapping repressive (H3 lysine 27 tri-methylation) and activating (H3 lysine 4 tri-methylation) histone modifications mark the promoters of more than 2,000 genes. To gain insight into the structure and function of
Katharina Rothe et al.
Blood, 123(23), 3622-3634 (2014-04-24)
Previous studies demonstrated that imatinib mesylate (IM) induces autophagy in chronic myeloid leukemia (CML) and that this process is critical to cell survival upon therapy. However, it is not known if the autophagic process differs at basal levels between CML
E M Michalak et al.
Cell death and differentiation, 15(6), 1019-1029 (2008-02-09)
The ability of p53 to induce apoptosis in cells with damaged DNA is thought to contribute greatly to its tumour suppressor function. P53 has been proposed to induce apoptosis via numerous transcriptional targets or even by direct cytoplasmic action. Two

Protocolos

Protocols for the Fmoc SPPS of Cysteine-containing Peptides

Overcome challenges in synthesis and disulfide bond formation with protocols for Fmoc solid-phase peptide synthesis of peptides with cysteine and methionine.

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