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Merck

700053P

Avanti

25-hydroxycholesterol-d6

Avanti Research - A Croda Brand

Sinónimos:

26,26,26,27,27,27-hexadeuterocholest-5-ene-3β,25-diol; 25-hydroxycholest-5-en-3-ol(d6)

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Fórmula empírica (notación de Hill):
C27H40D6O2
Número CAS:
Peso molecular:
408.69
MDL number:
NACRES:
NA.25
UNSPSC Code:
41141804
Assay:
>99% (TLC)
Form:
powder
Servicio técnico
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description

cholest-5-ene-3β,25-diol-d6

assay

>99% (TLC)

form

powder

packaging

pkg of 1 × 1 mg (700053P-1mg)

manufacturer/tradename

Avanti Research - A Croda Brand

shipped in

dry ice

storage temp.

−20°C

General description

25-hydroxycholesterol (25HC) is synthesized from cholesterol in the presence of oxygen and enzyme cholesterol 25-hydroxylase and the conversion is dependent on nicotinamide adenine dinucleotide phosphate (NADPH) during toll-like receptor activation. 25HC is catabolized to 7α-hydroxylated sterol. 25-hydroxycholesterol-d6 is the deuterated form of 25-hydroxycholesterol.

Application

25-hydroxycholesterol-d6 has been used as an internal standard:
  • in the quantification of 25-OHC in jejunum samples by liquid chromatography with tandem mass spectrometry (LC-MS-MS)
  • in plasma samples by liquid chromatography-mass spectrometry analysis
  • for calibration curve generation for plasma oxysterol quantification

Biochem/physiol Actions

25-hydroxycholesterol is a liver X receptor ligand. This bioactive lipid regulates immune response and macrophages production from monocytes. 25-HC serves as an antiviral effector and may have therapeutic potential. It reduces cholesterol biosynthesis by inactivating sterol regulatory element binding protein and inhibits HMG-CoA reductase (HMGCR). 25-HC may play a protective role in myocardial ischemia-reperfusion injury by inhibiting apoptosis and regulating mitogen-activated protein kinase (MAPK) pathway.

Packaging

5 mL Amber Glass Screw Cap Vial (700053P-1mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC


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Clase de almacenamiento

11 - Combustible Solids

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Stian Solheim et al.
The Journal of steroid biochemistry and molecular biology, 192, 105309-105309 (2019-02-20)
Oxysterols can contribute to proliferation of breast cancer through activation of the Estrogen Receptors, and to metastasis through activation of the Liver X Receptors. Endogenous levels of both esterified and free sidechain-hydroxylated oxysterols were examined in breast cancer tumours from
Editorial: 25-Hydroxycholesterol: a new life in immunology.
Jeffrey G McDonald et al.
Journal of leukocyte biology, 88(6), 1071-1072 (2010-12-03)
Ying Liu et al.
Journal of lipid research, 59(3), 439-451 (2018-01-05)
Cholesterol 25-hydroxylase (CH25H) catalyzes the production of 25-hydroxycholesterol (25-HC), an oxysterol that can play an important role in different biological processes. However, the mechanisms regulating CH25H expression have not been fully elucidated. In this study, we determined that CH25H is



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SKUGTIN
700053P-1MG04061835189175