10791156001

Endoproteinase Glu-C (V8 Protease)

from Staphylococcus aureus V8

• Synonym(s): V8 protease, protease v8
• Enzyme Commission number: 3.4.21.19 (BRENDA, IUBMB)

Properties

• formulario: lyophilized (salt-free)
• specific activity: 20 U/mg (Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at +37°C with casein as the substrate).); ~20 units/mg protein (At 25 °C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at 37 °C with casein as the substrate).)
• mol peso: 30 kDa
• envase: pkg of 2 mg
• manufacturer/tradename: Roche
• pH óptimo: 8.0-8.5

Description

Descripción general

Endoproteinase Glu-C is a Staphylococcal serine proteinase. Its inhibitors are DFP, α2-macroglobulin and TLCK.

Especificidad

Heat inactivation: Endoproteinase Glu-C is inactivated by boiling for ten minutes.

Aplicación

Use Endoproteinase Glu-C (V8 Protease) for protein structure analysis and for sequence analysis.

Envase

2 mg

Acciones bioquímicas o fisiológicas

Endoproteinase Glu-C specifically hydrolyzes peptide and ester bonds at the carboxylic side of Glu, or both Glu and Asp, depending on the buffer used.

Nota de preparación

Activator: The enzyme has its maximal activity in presence of SH-reagents
Working concentration: 1 to 5 mM
Working solution: Recommended solvent is 50 mM ammonium acetate pH 4.0 (2 mg/ml).
Storage conditions (working solution): -15 to -25 °C
The enzyme (2 mg/ml in 50 mM ammonium acetate, pH 4.0) is stable for at least one month, frozen in aliquots and thawed only once.

Almacenamiento y estabilidad

Store at 2 to 8 °C. (Store dry!)

Otras notas

For life science research only. Not for use in diagnostic procedures.

Safety Information

• pictogramas: GHS07,GHS08
• Palabra de señalización: Danger
• Frases de peligro: H315 - H319 - H334 - H335
• Consejos de prudencia: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Clasificaciones de peligro: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 1
• Punto de inflamabilidad F: does not flash
• Punto de inflamabilidad C: does not flash