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11417991001

Roche

SuRE/Cut Buffer H

solution, pkg of 5 × 1 mL

Synonym(s):
restriction enzyme buffer, SuRE/Cut Buffer for Restriction Enzymes

formulario

solution

Nivel de calidad

100

envase

pkg of 5 × 1 mL

manufacturer/tradename

Roche

enviado en

dry ice

temp. de almacenamiento

−20°C

Descripción general

The SuRE/Cut Buffer System consists of five optimized incubation buffers, 10x concentrated, for DNA restriction digest. Activity of all restriction enzymes has been determined in each buffer to select 100% activity or to calculate activity in double-digests.


Aplicación

SuRE/Cut Buffer H has been used to treat EcoRI and XhoI restriction enzymes to digest gel-purified reaction products and the pMX-IG vector. It has also been used to dilute the restriction enzyme Xanthomonas badrii I for the digestion of the genomic DNA of the E. coli colonies and the Salmonella Braenderup H2812 (ATCC BAA-664) marker.
The SuRE/Cut Buffer H has been used for digestion of genomic DNA using the restriction enzyme XbaI. It has been used for digestion of PCR products and plasmid using EcoRI and XhoI.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información legal

SuRE/Cut is a trademark of Roche

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 1

Punto de inflamabilidad F

No data available

Punto de inflamabilidad C

No data available

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Certificate of Origin

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Quotes and Ordering

Articles

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.

Related Content

Restriction Enzymes

Restriction endonucleases popularly referred to as restriction enzymes, are ubiquitously present in prokaryotes. The function of restriction endonucleases is mainly protection against foreign genetic material especially against bacteriophage DNA.

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