11418467001

Chymotrypsin Sequencing Grade

from bovine pancreas

• Synonym(s): chymotrypsin, protease
• Enzyme Commission number: 3.4.21.1 (BRENDA, IUBMB)

Properties

• origen biológico: bovine pancreas
• Nivel de calidad: 100
• ensayo: ≥90%
• formulario: lyophilized (salt-free)
• specific activity: ≥70 units/mg protein (at 25 °C, with ATEE as a substrate)
• mol peso: M_r 25 kDa
• envase: pkg of 4 × 25 μg
• manufacturer/tradename: Roche
• technique(s): protein sequencing: suitable
• pH óptimo: 7.0-9.0
• enviado en: wet ice
• temp. de almacenamiento: 2-8°C

Description

Descripción general

Chymotrypsin Sequencing Grade is isolated as a specific protease in pure form from bovine pancreas.

Especificidad

Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-terminial of Tyr, Phe, and Trp. Leu, Met, Ala, Asp, and Glu are cleaved at a lower rate. Acts also upon amides and esters of susceptible amino acids. The specificity of Chymotrypsin Sequencing Grade is tested with melittin as a substrate.

Aplicación

Use Chymotrypsin Sequencing Grade for the hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. It is suitable for peptide mapping, fingerprinting, and sequence analysis.

Calidad

Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.

Nota de preparación

Working concentration: The recommended amount of enzyme is 1/200 to 1/20 of the quantity of protein by weight.
Storage conditions (working solution): 2 to 8 °C
A solution in 1 mM HCl can be stored for up to one week at 2 to 8 °C.
Inhibitors: Aprotinin, DFP, PMSF, phenothiazine-N-carbonyl chloride, TPCK, ZPCK, α2-macroglobulin, α1-antitrypsin, soybean trypsin inhibitor, and chymostatin. No inhibition by APMSF.

Reconstitución

Dissolve lyophilized chymotrypsin sequencing grade in 1mM HCl. The proteins to be sequenced are dissolved in digestion buffer (100mM Tris-HCl, 10mM CaCl₂, pH 7.8).

Otras notas

For life science research only. Not for use in diagnostic procedures.

Safety Information

• pictogramas: GHS07,GHS08
• Palabra de señalización: Danger
• Frases de peligro: H315 - H319 - H334 - H335
• Consejos de prudencia: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Clasificaciones de peligro: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable