LMDH-RO

L-Malate Dehydrogenase (L-MDH)

from pig heart (mitochondrial)

• Número CE: 1.1.1.37 (BRENDA, IUBMB)
• Código UNSPSC: 12352202

Propiedades

• origen biológico: Porcine heart (mitochondrial)
• Nivel de calidad: 100
• Formulario: solution; suspension
• actividad específica: ~1200 units/mg protein (At 25 °C with oxaloacetate as the substrate.)
• envase: pkg of 1 mL (10127248001 solution); pkg of 1 mL (10127256001 suspension); pkg of 5 mL (10127914001suspension)
• fabricante / nombre comercial: Roche
• técnicas: activity assay: suitable
• color: white
• pH óptimo: 7.4-7.5(reduction of oxaloacetate); 9.2-9.5(oxidation of malate)
• solubilidad: water: miscible
• idoneidad: suitable for Western blot
• Nº de acceso NCBI: NM_001244153.1
• Nº de acceso UniProt: P00346
• aplicaciones: life science and biopharma
• actividad extraña: Fumarase <0.01%; GIDH 0.001%; GOT 0.001%; GPT 0.00185%; LDH <0.01%; NADH oxidase <0.001%
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: Porcine ... MDH2(397039)

Descripción

Descripción general

L-malate:NAD⁺ oxidoreductase
The L-malate dehydrogenase enzyme is a nuclear gene product that is synthesized with a 24-residue amino-terminal signal peptide. This peptide is proteolytically cleaved during the translocation of the enzyme to the mitochondrial matrix.[1]

Aplicación

The enzyme L-malate dehydrogenase from pig heart has been used to measure PEPCK (phosphoenolpyruvate carboxykinase) activity. The oxaloacetate, produced by PEPCK, is reduced to malate via the oxidation of NADH, which in turn is measured at 340 nm using a spectrophotometer.[2] The enzyme has also been used to measure oxaloacetate by measuring the reduction in NADH spectroscopically at 340 nm.[3]

Acciones bioquímicas o fisiológicas

The enzyme L-malate dehydrogenase from pig heart catalyzes the oxidation of L-malate to oxaloacetate. The enzyme is an NAD-dependent mitochondrial dehydrogenase that functions in the tricarboxylic acid cycle. It is a component of the malate-aspartate shuttle that transports reducing equivalents across the inner mitochondrial membrane in the form of malate.[1]

Calidad

Contaminants: <0.002% GOT, <0.01% fumarase and LDH, each luM each, <0.02% HK, <0.002% PGI

Forma física

Solution in 50% glycerol (v/v), pH approximately 7

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Nota de preparación

Activator: – phosphate
– arsenate
– Mg²⁺
– Zn²⁺

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): No data available
• Punto de inflamabilidad (°C): No data available