04055

Gelatin from porcine skin

powder, suitable for microbiology

• CAS Number: 9000-70-8
• Número de EC: 232-554-6
• Número MDL: MFCD00081638
• NACRES: NA.85

Properties

• origen biológico: Porcine
• Nivel de calidad: 200
• esterilidad: non-sterile
• formulario: powder
• impurezas: ≤0.005% heavy metals (as Pb); ≤0.01% peroxides (as H₂O₂); ≤0.05% sulfur dioxide (SO₂)
• residuo de ign.: ≤2%
• pérdida: ≤15% loss on drying
• pH: 3.8-7.6 (50 °C, 67 mg/mL in H₂O)
• fuerza de gel: 170-190 g Bloom67 mg/ml water
• trazas de catión: As: ≤1 mg/kg
• application(s): microbiology

Description

Aplicación

This product is recommended for use as a cell culture substratum at 1-5 μg/cm² or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Componentes

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Precaución

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Nota de preparación

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g.

Safety Information

• Código de clase de almacenamiento: 13 - Non Combustible Solids
• WGK: nwg
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)