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04732

Supelco

2,4-Dinitrophenylhydrazine

for HPLC derivatization, LiChropur, moistened with 35% water, ≥99.0% (HPLC)

Fórmula lineal:
(O2N)2C6H3NHNH2
Número de CAS:
Peso molecular:
198.14
Beilstein:
615586
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.22

Quality Level

grade

for HPLC derivatization

assay

≥99.0% (HPLC)

quality

LiChropur
moistened with 35% water

application(s)

HPLC: suitable

mp

197-200 °C (lit.)

SMILES string

NNc1ccc(cc1[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C6H6N4O4/c7-8-5-2-1-4(9(11)12)3-6(5)10(13)14/h1-3,8H,7H2

InChI key

HORQAOAYAYGIBM-UHFFFAOYSA-N

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Application

2,4-Dinitrophenylhydrazine may be used as a derivatization agent in the analysis of aldehydes and ketones using liquid chromatography coupled with atmospheric pressure photoionization-mass spectrometry (LC-APPI-MS).

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Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Certificado de Análisis

Certificado de origen

The determination of dehydroascorbic acid and ascorbic acid in plant tissues by the 2, 4-dinitrophenylhydrazine method.
Roe JH, et al.
The Journal of Biological Chemistry, 152, 511-517 (1944)
Determination of aldehydes and ketones using derivatization with 2, 4-dinitrophenylhydrazine and liquid chromatography?atmospheric pressure photoionization-mass spectrometry.
van Leeuwen SM, et al.
Journal of Chromatography A, 1058(1-2), 107-112 (2004)
María Tabernero et al.
Food & function, 5(7), 1556-1563 (2014-05-24)
Hydroxytyrosol (HT), a virgin olive oil phenolic phytochemical with proven health benefits, has been used to generate new lipophilic antioxidants to preserve fats and oils against autoxidation. The aim of this work is to comparatively evaluate the physiological effects of...
Jorge Gutiérrez et al.
Journal of experimental botany, 65(12), 3081-3095 (2014-04-12)
Light-grown Arabidopsis thaliana cell suspension culture (ACSC) were subjected to mild photooxidative damage with Rose Bengal (RB) with the aim of gaining a better understanding of singlet oxygen-mediated defence responses in plants. Additionally, ACSC were treated with H2O2 at concentrations...
Maurizio Vitadello et al.
The Journal of physiology, 592(12), 2637-2652 (2014-04-09)
Antioxidant administration aimed to antagonize the development and progression of disuse muscle atrophy provided controversial results. Here we investigated the effects of curcumin, a vegetal polyphenol with pleiotropic biological activity, because of its ability to upregulate glucose-regulated protein 94 kDa...

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