Merck
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50484

Sigma-Aldrich

Methylene Blue solution

suitable for microbiology

Synonym(s):
Loeffler’s Methylene Blue, Ehrlich’s reagent III
Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
Molecular Weight:
319.85
Beilstein:
3641570
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.47

caducidad

limited shelf life, expiry date on the label

concentración

0.5% in H2O (0.5 g/100 mL)

technique(s)

microbe id | staining: suitable

application(s)

microbiology

idoneidad

Bacillus spp.
Corynebacterium spp.
bacteria

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

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Descripción general

Methylene Blue was earlier recognized as a good nuclear stain, the staining solutions are always either aged or alkalized, if not both. More importantly it is an excellent stain for ribonucleic acid, which is the important stainable constituent of bacterial bodies, of lymphocyte and hemoportoal cytoplasm, and of nerve cell tigroid granules. It can also be employed in capsule stain. The capsule stain begins as a negative stain leaving the organisms colorless. The capsule are clearly seen as halo surrounding the bacterial cell. Another staining procedure is performed with nigrosin (Cat. No. 43925).

Componentes

reagent contains:
methylene blue 0.5 g
distilled water 100 ml

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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