63069

Magnesium chloride solution

BioUltra, for molecular biology, ~1 M in H₂O

• Synonym(s): Magnesium Chloride hydrate, Magnesium chloride (MgCl₂)hexahydrate, MgCl₂
• Linear Formula: MgCl₂
• CAS Number: 7786-30-3
• Molecular Weight: 95.21
• Número MDL: MFCD00011106
• ID de la sustancia en PubChem: 329759772
• NACRES: NA.26

Properties

• grado: for molecular biology
• Nivel de calidad: 200
• Línea del producto: BioUltra
• concentración: ~1 M in H₂O
• impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; ≤0.0002% total Nitrogen (N)
• pH: 5.0-7.5 (25 °C, 1 M in H₂O)
• densidad: 1.08 g/mL at 20 °C
• trazas de anión: phosphate (PO₄^3-): ≤10 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• trazas de catión: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤10 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤50 mg/kg; Li: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Na: ≤500 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: 1 M in H₂O
• Absorción UV: λ: 260 nm A_max: 0.05; λ: 280 nm A_max: 0.03
• SMILES string: Cl[Mg]Cl
• InChI: 1S/2ClH.Mg/h2*1H;/q;;+2/p-2
• InChI key: TWRXJAOTZQYOKJ-UHFFFAOYSA-L

Description

Descripción general

Magnesium chloride is widely used in polymerase chain reactions (PCR) buffers to supply magnesium ions. The concentration of Mg²⁺ is the most critical parameter that impacts both the efficiency and specificity of PCR when total genomic DNA is used, synthesis of long products, or during multiplex reactions. Elevated concentrations of Mg²⁺ enhance the stability of primer-template duplexes and the efficiency of amplification.

Aplicación

Magnesium chloride solution has been used as a component of nuclei isolation buffer for in situ fixations and isolation of nuclei from Kupffer cells (or other liver cells).

Magnesium chloride solution has been used in the reaction mix of reverse transcriptase enzyme, PFV RT. It has been used in the preparation of artificial cerebrospinal fluid (ACSF).

Envase

25, 100, 500 mL

Safety Information

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves