A0169

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A0169

Agarose

Type I-A, low EEO

All Photos(8)

Synonym(s):

3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE

CAS Number:

9012-36-6

Número de EC:

232-731-8

Número MDL:

MFCD00081294

ID de la sustancia en PubChem:

24890407

NACRES:

NA.25

Nivel de calidad

200

tpo

Type I-A

formulario

powder

impurezas

≤7% water

ceniza

≤0.5%

turbidez

≤4 NTU (1.5% gel)

ELECTROENDÓSMOSIS (EEO)

0.09-0.13

mp

86.5-89.5 °C

temperatura de transición

gel point 36 °C ±1.5 °C (1.5% gel)

fuerza de gel

≥1200 g/cm² (1% gel)

trazas de anión

sulfate (SO₄^2-): ≤0.20%

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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Agarose: Properties and Research Applications

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This Item	A9539	A4718	A4679
Sigma-Aldrich A0169 Agarose	Sigma-Aldrich A9539 Agarose	Sigma-Aldrich A4718 Agarose	Sigma-Aldrich A4679 Agarose
form powder	form powder	form powder	form powder
impurities ≤7% water	impurities ≤10% moisture content	impurities -	impurities ≤7% water
EEO 0.09-0.13	EEO 0.09-0.13	EEO ≤0.05	EEO 0.09-0.13
transition temp gel point 36 °C ±1.5 °C (1.5% gel)	transition temp gel point 36 °C ±1.5 °C (1.5% gel)	transition temp gel point ≤35 °C (3% gel)	transition temp gel point 34-38 °C (1.5% gel)
gel strength ≥1200 g/cm² (1% gel)	gel strength ≥1200 g/cm² (1% gel)	gel strength -	gel strength ≥1200 g/cm² (1% gel)
anion traces sulfate (SO₄^2-): ≤0.20%	anion traces sulfate (SO₄^2-): ≤0.15%	anion traces -	anion traces sulfate (SO₄^2-): ≤0.20%

Descripción general

Agarose, a natural polymer is obtained from sea algae. This neutral linear polysaccharide is made up of d-galactose and 3,6-anhydro-l-galactose. These are connected alternatively with the help of glycosidic bonds. Agarose has high significance in gel electrophoresis and gel chromatography.

Aplicación

Agarose has been used:

as a component in the preparation of tumor tissue to embed the cell material
in the sample preparation for quantum dot analysis
in the preparation of agarose-solidified medium to study the effect of medium solidification on the culture of isolated ovules

Acciones bioquímicas o fisiológicas

Agarose is used as bioink, because of its characteristic properties such as, biocompatibility, mechanical strength and gelling ability at low temperature.

Nota de análisis

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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