A2790

Agarose

Wide range, for molecular biology

• Synonym(s): 3,6-Anhydro-α-L-galacto-β-D-galactan
• CAS Number: 9012-36-6
• Número de EC: 232-731-8
• Número MDL: MFCD00081294
• ID de la sustancia en PubChem: 24890692
• NACRES: NA.25

Properties

• origen biológico: algae (marine)
• Nivel de calidad: 200
• grado: for molecular biology
• formulario: powder
• technique(s): electrophoresis: suitable
• ELECTROENDÓSMOSIS (EEO): ≤0.15
• mp: ≤65 °C
• temperatura de transición: gel point ≤35 °C
• trazas de anión: sulfate (SO₄^2-): ≤0.15%
• actividad extraña: DNase, RNase, none detected
• InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
• InChI key: MJQHZNBUODTQTK-WKGBVCLCSA-N

Description

Aplicación

Wide range agarose is capable of separating DNA fragments from 50-1,000 bp on a single 3% agarose gel.

Nota de análisis

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Safety Information

• Código de clase de almacenamiento: 13 - Non Combustible Solids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)