A7431

Agarose

BioReagent, for molecular biology, Wide range/Standard 3:1

• Synonym(s): 3,6-Anhydro-α-L-galacto-β-D-galactan
• CAS Number: 9012-36-6
• Número de EC: 232-731-8
• Número MDL: MFCD00081294
• ID de la sustancia en PubChem: 24891233
• NACRES: NA.25

Properties

• origen biológico: algae (marine)
• Nivel de calidad: 200
• grado: for molecular biology
• Línea del producto: BioReagent
• formulario: powder
• technique(s): electrophoresis: suitable
• ELECTROENDÓSMOSIS (EEO): ≤0.13
• mp: ≤90 °C
• temperatura de transición: gel point 32.5-38 °C (4% gel)
• fuerza de gel: ≥1200 g/cm² (4% gel)
• trazas de anión: sulfate (SO₄^2-): ≤0.15%
• actividad extraña: DNase, RNase, NICKase, none detected
• InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
• InChI key: MJQHZNBUODTQTK-WKGBVCLCSA-N

Description

Aplicación

Specially formulated to form strong, flexible gels for separation of small (≤1 kb) PCR products, DNA and RNA. Stronger gels make gel-handling easier. Sharp resolution is produced with DNA fragments ranging from 10-1500 bp.

Nota de análisis

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Safety Information

• Código de clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)