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Merck

B4501

Sigma-Aldrich

Biotina

≥99% (HPLC), lyophilized powder

Sinónimos:

D-Biotina, Bios II, Coenzima R, Vitamina H, Vitaminas B7

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100 MG
MXP 659.00
500 MG
MXP 1,430.00
1 G
MXP 2,248.00
5 G
MXP 9,687.00
10 G
MXP 16,011.00
25 G
MXP 35,043.00

MXP 659.00

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Fórmula empírica (notación de Hill):
C10H16N2O3S
Número CAS:
58-85-5
Peso molecular:
244.31
Beilstein/REAXYS Number:
86838
EC Number:
200-399-3
MDL number:
MFCD00005541
UNSPSC Code:
12352201
eCl@ss:
34058011
PubChem Substance ID:
24891778
NACRES:
NA.21

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biological source

synthetic (organic)

Quality Level

300

assay

≥99% (HPLC)

form

lyophilized powder

technique(s)

HPLC: suitable

color

white to off-white

mp

231-233 °C (lit.)

solubility

ammonium hydroxide: 50 mg/mL (2 M)

storage temp.

2-8°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2

InChI

1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1

InChI key

YBJHBAHKTGYVGT-ZKWXMUAHSA-N

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1 of 4

Este artículo
B46399182714400
Biotina ≥99% (HPLC), lyophilized powder

Sigma-Aldrich

B4501

Biotina

Biotina powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

Sigma-Aldrich

B4639

Biotina

Biotina certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

Supelco

91827

Biotina

Biotina ≥99.0% (T)

Sigma-Aldrich

14400

Biotina

technique(s)

HPLC: suitable

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, gas chromatography (GC): suitable

technique(s)

-

assay

≥99% (HPLC)

assay

≥99%

assay

-

assay

≥99.0% (T)

biological source

synthetic (organic)

biological source

-

biological source

-

biological source

synthetic

form

lyophilized powder

form

powder

form

-

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

solubility

ammonium hydroxide: 50 mg/mL (2 M)

solubility

H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.)

solubility

-

solubility

alcohol: soluble 80mg/100 ml at 25 °C, water: soluble 22mg/100 ml at 25 °C, 0.1 M NaOH: 10 mg/mL at 25 °C, clear, colorless, H2O: very soluble, organic solvents: insoluble

General description

Biotin is a water-soluble vitamin. Mammals depend on biotin via plant and microbial dietary sources.[1] Biotin consists of two rings that are attached to valeric acid moiety as a side chain. One of the rings is attached to ureido group and the other ring is the tetrahydrothiophene group.[2]

Application

Biotin may be used to elute proteins from avidin/streptavidin resins. Biotin has been used:
  • for culturing of oligodendrocytes.[3][4]
  • as a vitamin supplement for the growth of Bacillus species.[5]
  • for blocking endogenous biotin during immunohistology procedures.[6][7]

Biochem/physiol Actions

Biotin is a small molecule and its presence with biologically active macromolecules does not affect the biological function of the macromolecule. The biotin-(strept)avidin system are is required for various applications. Biotin also works as a cofactor/coenzyme of carboxylating enzymes, such as pyruvate carboxylase, acetyl-CoA carboxylases 1 and 2, 3-methylcrotonyl-CoA carboxylase (MCC), and propionyl-CoA carboxylase (PCC).[1] It catalyzes the formation of oxaloacetate from pyruvate and CO2. Biotin conjugation with avidin or streptavidin helps in linking the target molecule (antibody, nucleotide, protein A, etc.) with the labeling system (enzyme, fluorescent, chemiluminescent probes). This complex is used for many detection systems, such as immunoassays, DNA hybridization assays, immunohistochemistry, and flow cytometry. This approach is also applied for to the purification and characterization of various molecules of interest. Biotin is involved in the epigenetic regulation of genes, cell signaling, and the structure of chromatin.[1]

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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Clase de almacenamiento

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000496252
0000496259
0000496252
0000496259
0000494377

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The biotin-(strept)avidin system: principles and applications in biotechnology.
Diamandis EP and Christopoulos TK
Clinical Chemistry, 37, 625-625 (1991)
Isolation and culture of rat and mouse oligodendrocyte precursor cells.
Chen Y et al.
Nature Protocols, 2, 1044-1044 (2007)
Lone Frydelund-Larsen et al.
Experimental physiology, 92(1), 233-240 (2006-10-13)
Exercise induces a marked increase in interleukin-8 (IL-8) mRNA and protein expression within skeletal muscle fibres. Interleukin-8 belongs to a subfamily of CXC chemokines containing a Glu-Leu-Arg (ELR) motif. CXC chemokines with ELR motifs are potent angiogenic factors in vivo
Biotin: biochemical, physiological and clinical aspects
Said H M
Sub-Cellular Biochemistry, 56, 1-19 (2012)
Yuan He et al.
Nature, 530(7590), 354-357 (2016-01-28)
Inflammasomes are intracellular protein complexes that drive the activation of inflammatory caspases. So far, four inflammasomes involving NLRP1, NLRP3, NLRC4 and AIM2 have been described that recruit the common adaptor protein ASC to activate caspase-1, leading to the secretion of
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Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Hi, I bought Biotin with Cat. number B4501, I want to know how can I prepare biotin solution?

    1 answer

    1. Please view the 'Preparation Instructions' in the product Datasheet below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/565/952/b4501dat.pdf

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