Merck
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H23830

Sigma-Aldrich

HEPES

reagent grade, ≥99% (titration), crystalline

Synonym(s):
Ácido 4-(2-hidroxietil)piperacina-1-etanosulfónico, Ácido N-(2-hidroxietil)piperacina-N′-(2-etanosulfónico)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
Número MDL:

origen biológico

synthetic

Nivel de calidad

grado

reagent grade

Análisis

≥99% (titration)

formulario

crystalline

useful pH range

6.8-8.2

pKa (25 °C)

7.5

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

Inchi Key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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H4034H7523H3375
HEPES reagent grade, ≥99% (titration), crystalline

Sigma-Aldrich

H23830

HEPES

HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

Sigma-Aldrich

H4034

HEPES

HEPES BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Sigma-Aldrich

H7523

HEPES

HEPES ≥99.5% (titration)

Sigma-Aldrich

H3375

HEPES

grade

reagent grade

grade

BioPerformance Certified

grade

-

grade

-

assay

≥99% (titration)

assay

≥99.5% (titration)

assay

≥99.5% (titration)

assay

≥99.5% (titration)

form

crystalline

form

crystalline powder

form

powder

form

crystalline powder

Quality Level

200

Quality Level

400

Quality Level

400

Quality Level

400

biological source

synthetic

biological source

-

biological source

-

biological source

-

useful pH range

6.8-8.2

useful pH range

6.8-8.2

useful pH range

6.8-8.2

useful pH range

6.8-8.2

Descripción general

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used as a buffer in cell culture media. HEPES finds its usage in in vitro experiments on Mg.

Aplicación

HEPES has been used:
  • as a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo
  • as a component of Hanks′ solution used in the degradation testing of Iron
  • as a component of HEPES medium in the transfection of cell culture with plasmids
  • along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells
  • as a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies

Características y beneficios

  • Broad buffer range between pH 6.8 to 8.2
  • Zwitterionic at biological pH
  • High solubility and stability
  • Low UV and visible light spectrum absorbance
  • Negligible metal ion binding
  • Suitable for cell culture, electrophoresis, in vitro methods, etc.

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable


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