H3136

Hematoxylin

certified by the Biological Stain Commission

• Synonym(s): Natural Black 1
• Empirical Formula (Hill Notation): C₁₆H₁₄O₆ · xH₂O
• CAS Number: 517-28-2
• Molecular Weight: 302.28 (anhydrous basis)
• Número de índice de color: 75290
• Beilstein: 91400
• Número de EC: 208-237-3
• Número MDL: MFCD00078111
• ID de la sustancia en PubChem: 24895554
• NACRES: NA.47

Properties

• grado: certified by the Biological Stain Commission
• Nivel de calidad: 200
• formulario: powder
• mp: 200 °C (dec.) (lit.)
• solubilidad: ethanol: 1 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: room temp
• SMILES string: Oc1cc2C[C@@]3(O)COc4c(O)c(O)ccc4[C@H]3c2cc1O
• InChI: 1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m0/s1
• InChI key: WZUVPPKBWHMQCE-XJKSGUPXSA-N
• Gene Information: rat ... Adora1(29290) , Adora2a(25369)

Description

Descripción general

Hematoxylin is a basophilic dye extracted from the tree Heamatoxylum campechianum. It specifically binds to acidic compounds and thus is useful for staining cell nuclei which contain acidic substances: DNA and RNA. Hematoxylin, when oxidized forms hematein, which has thick pale blue purple colour. Hematoxylin is often used along with eosin, in histological study under light microscope.

Aplicación

Hematoxylin has been used:

• to visualize anatomical features and to detect nucleus locus coeruleus adjacent to the periaqueductal gray region in brainstem samples
• as a nuclear counterstain to visualize the nucleus, adipocytes were counterstained with a hematoxylin reagent
• to stain lung tissue for histological analysis

Idoneidad

Certified for use in Weigert′s iron hematoxylin, Mayer′s hemalum, Harris hemalum and in Heidenhain′s technique

Safety Information

• pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H319
• Consejos de prudencia: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Clasificaciones de peligro: Eye Irrit. 2
• Código de clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves