Merck
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P3504

Sigma-Aldrich

Ponceau S

BioReagent, suitable for electrophoresis

Sinónimos:
3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt
Empirical Formula (Hill Notation):
C22H12N4Na4O13S4
Número de CAS:
Peso molecular:
760.57
Número de índice de color:
27195
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.47

Nivel de calidad

200

línea de producto

BioReagent

formulario

powder

technique(s)

electrophoresis: suitable

solubilidad

H2O: 1 mg/mL

ε (extinction coefficient)

≥330000 at 518-522 nm in deionized water at 0.015 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

InChI key

KQHKSGRIBYJYFX-WNDMICSESA-J

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Descripción general

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection.
The following are two common stain formulations:
0.1% Ponceau S (w/v) in 5% acetic acid
2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid

Aplicación

Ponceau S has been used for membrane staining (Western blotting.

Envase

10, 50, 100 g in glass bottle

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

Certificado de Análisis

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Certificado de origen

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